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. 1978 May;26(2):221-5.
doi: 10.1128/JVI.26.2.221-225.1978.

Transfection of Streptococcus pneumoniae with bacteriophage DNA

C Ronda, R Lopez, A Tomasz, A Portoles

Transfection of Streptococcus pneumoniae with bacteriophage DNA

C Ronda et al. J Virol. 1978 May.

Abstract

It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).

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