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. 2016 Feb;22(2):292-4.
doi: 10.3201/eid2202.151395.

Ebola Virus RNA Stability in Human Blood and Urine in West Africa's Environmental Conditions

Frédéric Janvier, Deborah Delaune, Thomas Poyot, Eric Valade, Audrey Mérens, Pierre E Rollin, Vincent Foissaud

Ebola Virus RNA Stability in Human Blood and Urine in West Africa's Environmental Conditions

Frédéric Janvier et al. Emerg Infect Dis. 2016 Feb.

Abstract

We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West Africa's environmental conditions. In blood, RNA was stable for at least 18 days when initial cycle threshold values were <30, but in urine, RNA degradation occurred more quickly.

Keywords: Ebola; Ebola virus; RNA stability; West Africa; blood; human samples; rRT-PCR; urine; viruses.

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Figures

Figure
Figure
Stability of Ebola virus RNA in A) EDTA plasma and B) urine samples from patients in Guinea, as measured by real-time reverse transcription PCR. EDTA plasma and urine were processed immediately after receipt at a laboratory and left at room temperature (22°C–29°C; 50%–80% relative humidity) for various periods before the PCR analysis. Average cycle threshold values ± SD for individual samples are shown.
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