Corrigendum

Abstract

Pyroptotic neuronal cell death mediated by the AIM2 inflammasomeAdamczak SE, de Rivero Vaccari JP, Dale G, Brand FJ 3rd, Nonner D, Bullock MR, Dahl GP, Dietrich WD, Keane RWJournal of Cerebral Blood Flow & Metabolism 2014; 34: 621-629.

Figure 5.

Neuronal pyroptosis is inhibited by probenecid and the pannexin 1 inhibitor BB FCF. In panel A, embryonic neonatal cortical neurons were stimulated with poly(dA:dT)) in the presence or absence of YVAD, probenecid, or BB FCF, and stained with a small (629 Da) membrane impermeable dye, YO-PRO®-1, (green) and a larger (1293 Da) membrane impermeable dye, Eth-D2, (red) to identify neurons with discrete membrane pores. Hoechst stain (blue) shows total cells in each field. Small, 1–2 nm, caspase-1-dependent pores form in a subpopulation (∼20%) of cortical neurons stimulated with poly(dA:dT). Selective inhibition of pannexin 1 by either probenecid or BB FCF abolishes pore formation and uptake of the smaller dye following activation of the AIM2 inflammasome.