Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Randomized Controlled Trial
. 2015 Nov-Dec;23(6):562-70.
doi: 10.1590/1678-775720150239.

Effect of 10% fluoride on the remineralization of dentin in situ

Mozhgan Bizhang  1 , Sabine Kaleta-Kragt  1 , Preeti Singh-Hüsgen  2 , Markus Jörg Altenburger  3 , Stefan Zimmer  1
Affiliations
Randomized Controlled Trial

Effect of 10% fluoride on the remineralization of dentin in situ

Mozhgan Bizhang et al. J Appl Oral Sci. 2015 Nov-Dec.

Abstract

Objective The purpose of this randomized, cross-over, in situ study was to determine the remineralization of demineralized dentin specimens after the application of a 10% fluoride (F-) or a 1% chlorhexidine-1% thymol (CHX-thymol) varnish. Material and Methods Twelve individuals without current caries activity wore removable appliances in the lower jaw for a period of four weeks. Each appliance contained four human demineralized dentin specimens fixed on the buccal aspects. The dentin specimens were obtained from the cervical regions of extracted human third molars. After demineralization, half the surface of each specimen was covered with a nail varnish to serve as the reference surface. The dentin specimens were randomly assigned to one of the three groups: F-, CHX-thymol, and control (no treatment). Before the first treatment period and between the others, there were washout periods of one week. After each treatment phase, the changes in mineral content (vol% µm) and the lesion depths (µm) of the dentin slabs were determined by transverse microradiography (TMR). Data analysis was accomplished by the Kruskal-Wallis test and the Mann-Whitney U test (p<0.05). Results The medians (25th/75th percentile) of integrated mineral loss were 312.70 (203.0-628.7) for chlorhexidine varnish, 309.5 (109.8-665.8) for fluoride varnish, and -346.9 (-128.7 - -596.0) for the control group. The medians (25th/75th percentile) of lesion depth were 13.6 (5.7-34.5) for chlorhexidine varnish, 16.5 (5.6-38.1) for fluoride varnish, and -14.2 (-4.5- -32.9) for the control group. Use of the 10% F- or 1% CHX-1% thymol varnishes resulted in significantly decreased mineral loss and lesion depth in dentin when compared with the control group. There were no statistically significant differences among the test groups. Conclusions Within the limitations of this study, the results suggest that the effect of the treatment of demineralized dentin with 10% F- or 1% CHX-1% thymol is better than without any treatment.

PubMed Disclaimer

Conflict of interest statement

CONFLICT OF INTEREST AND SOURCE OF FUNDING STATEMENT

All authors declare that they have no conflict of interests. The study was supported by VOCO GmbH (Cuxhaven, Germany). The company provided funding for materials, compensation for participants, research equipment and supplies. The company had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Figures

Figure 1
Figure 1. Study design
Figure 2
Figure 2. The intraoral appliance (a) and dentin specimen (b)
Figure 3
Figure 3. Median of the mineral content (Vol%∙µm) in a thin section of the dentin specimens in situ (***p<0.001; n.s.: no statistically significant differences)
Figure 4
Figure 4. Median of the reduction in lesion depth (µm) in a thin section of the dentin specimens in situ (***p<0.001; n.s.: no statistically significant differences)
Figure 5
Figure 5. Profile examples of the mineral volume percentage
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Addy M, Newcombe RG. Statistical versus clinical significance in periodontal research and practice. Periodontol 2000. 2005;39:132–144. - PubMed
    1. Baysan A, Lynch E, Ellwood R, Davies R, Petersson L, Borsboom P. Reversal of primary root caries using dentifrices containing 5,000 and 1,100 ppm fluoride. Caries Res. 2001;35:41–46. - PubMed
    1. Biesbrock AR, Bartizek RD, Gerlach RW, Jacobs SA, Archila L. Dose response efficacy of sodium fluoride dentifrice at 9 and 21 months with supervised brushing. Am J Dent. 2003;16:305–312. - PubMed
    1. Bizhang M, Chun YH, Winterfeld MT, Altenburger MJ, Raab WH, Zimmer S. Effect of a 5000 ppm fluoride toothpaste and a 250 ppm fluoride mouth rinse on the demineralisation of dentin surfaces. BMC Res Notes. 2009;2: - PMC - PubMed
    1. Bizhang M, Seemann R, Römhild G, Chun YH, Umland N, Lang H, et al. Effect of a 40% chlorhexidine varnish on demineralization of dentin surfaces in situ. Am J Dent. 2007;20:193–197. - PubMed

Publication types