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. 2016:1406:53-9.
doi: 10.1007/978-1-4939-3444-7_4.

In Situ Hybridization of Breast Cancer Markers

Li Min  1 , Chengchao Shou  2
Affiliations

In Situ Hybridization of Breast Cancer Markers

Li Min et al. Methods Mol Biol. 2016.

Abstract

In situ hybridization is an important technique in breast cancer research, which is widely applied in detection of specific nucleic acid sequences. Here, we describe the detailed protocol of fluorescence in situ hybridization and chromogenic in situ hybridization in detection of gene HER2/neu amplification in breast cancer tissues.

Keywords: Breast cancer; CISH; FISH; HER2; In situ hybridization.

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