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. 2016 Jan 25:10:15-20.
doi: 10.4137/GRSB.S35368. eCollection 2016.

Expression and Presence of OPG and RANKL mRNA and Protein in Human Periodontal Ligament with Orthodontic Force

Liliana Otero  1 , Dabeiba Adriana García  2 , Liseth Wilches-Buitrago  3
Affiliations

Expression and Presence of OPG and RANKL mRNA and Protein in Human Periodontal Ligament with Orthodontic Force

Liliana Otero et al. Gene Regul Syst Bio. .

Abstract

Objective: The objective of this study is to investigate the expression and concentration of ligand receptor activator of NFkB (RANKL) and osteoprotegerin (OPG) in human periodontal ligament (hPDL) with orthodontic forces of different magnitudes.

Methods: Right premolars in 32 patients were loaded with 4oz or 7oz of orthodontic force for 7 days. Left first premolars were not loaded. After 7 days, premolars were extracted for treatment as indicated. OPG and RANKL mRNA expressions were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and ELISA was used to assess OPG and RANKL protein concentration in compression and tension sides of PDL. Data were subjected to analysis of variance and Tukey tests.

Results: There was statistically significant difference in RANKL concentration on comparing control teeth with tension and compression sides of the experimental teeth (P < 0.0001). The expression of mRNA RANKL was increased in the tension and compression sides with 4oz (P < 0.0001). OPG did not show statistically significant association with any group. Changes in RANKL/OPG protein ratio in experimental and control groups showed statistically significant difference (P < 0.0001).

Conclusions: RANKL protein levels are elevated in hPDL loaded with orthodontic forces, suggesting that RANKL protein contributes to bone modeling in response to the initial placement of orthodontic force.

Keywords: OPG; RANKL; bone modeling; orthodontic forces.

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Figures

Figure 1
Figure 1
Experimental appliance. The force applied to the premolars in each group was measured using Dontrix (Dentspaly®).
Figure 2
Figure 2
mRNA expression levels of RANKL and OPG. (A) Comparison of mRNA expression of RANKL in tension/compression sides of the experimental teeth loaded with 4oz of force (P < 0.0001) and control teeth. (B) Comparison of mRNA expression of OPG in tension/compression sides of the experimental teeth loaded with 4oz and 7oz of force (P = 0.31) and control teeth.
Figure 3
Figure 3
RANKL and OPG protein levels. (A) Comparison of RANKL protein in tension/compression sides of the experimental teeth loaded with 4oz and 7oz of force (P < 0.0001) and control teeth. (B) Comparison of OPG protein in tension/compression sides of the experimental teeth loaded with 4oz and 7oz of force (P = 0.07) and control teeth.
Figure 4
Figure 4
Changes in RANKL/OPG protein (P < 0.0001) and mRNA (P > 0.05) ratios in experimental and control groups.
See this image and copyright information in PMC

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