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. 2015 Nov 1;8(11):13806-13.
eCollection 2015.

Influence of interleukin-1 beta gene polymorphisms on the risk of myocardial infarction and ischemic stroke at young age in vivo and in vitro

Bo Yang  1 , Hua Zhao  1 , Bin X  1 , Ya-Bin Wang  1 , Jian Zhang  1 , Yu-Kang Cao  1 , Qing Wu  1 , Feng Cao  1
Affiliations

Influence of interleukin-1 beta gene polymorphisms on the risk of myocardial infarction and ischemic stroke at young age in vivo and in vitro

Bo Yang et al. Int J Clin Exp Pathol. .

Abstract

In this study, by using vivo and vitro model, we assessed whether interleukin (IL)-1beta gene polymorphisms influence on the risk of myocardial infarction and ischemic stroke at young age. 147 patients (age < 45 years) with a first episode of MI and 56 patients (age < 45 years) with first-ever cerebral ischemia consecutively were admitted to this study from the Department of Chinese PLA General Hospital. Meanwhile, 91 normal volunteers without MI or stroke were deeded as control group and greed to give blood samples for DNA analysis and biochemical measurements by written informed consent. IL-1β-511 wild type (WT, CC) and SNP (TT) were established and transfected into Rat myocardial H9c2 cell and Mouse brain endothelial bEND.3 cells. In Young Age MI or stroke patients, the IL-1β levels of patients with 511CC are higher than that of patients with 511TT. In our study, NF-κB miRNA, iNOS activity, NF-κB, iNOS and Bax protein expressions of MI-induced H9c2 cell or stroke-induced bEND.3 cells in IL-1β-511TT group were lower than those of IL-1β-511CC. Additionally, the protein expression of MMP-2 of MI-induced H9c2 cell or stroke-induced bEND.3 cells in IL-1β-511TT group were higher than that of IL-1β 511CC group. In conclusion, our data indicate that IL-1β-511TT/CC influence on the risk of myocardial infarction and ischemic stroke at young age through NF-κB, iNOS, MMP-2 and Bax.

Keywords: IL-1β gene polymorphisms; IL-1β-511TT/CC; ischemic stroke; myocardial infarction.

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Figures

Figure 1
Figure 1
IL-1β-511 WT and SNP and Risk of MI at Young Age. CC, IL-1β-511CC (WT/WT); CT, IL-1β-511 CT (WT/SNP); TT, IL-1β-511 TT (SNP/SNP). ##P < 0.01 compared with CC group; #P < 0.01 compared with CT group.
Figure 2
Figure 2
The effect of IL-1β-511 WT and SNP on NF-κB in MI-induced cell. NF-κB activity (A), NF-κB protein expression using Western blotting assays (B) and NF-κB protein expression using Statistical Analysis (C). ##P < 0.01 compared with CC group.
Figure 3
Figure 3
The effect of IL-1β-511 WT and SNP on iNOS in MI-induced cell. iNOS activity (A), iNOS protein expression using Western blotting assays (B) and iNOS protein expression using Statistical Analysis (C). ##P < 0.01 compared with CC group.
Figure 4
Figure 4
The effect of IL-1β-511 WT and SNP on MMP-2 in MI-induced cell. MMP-2 protein expression using Western blotting assays (A) and iNOS protein expression using Statistical Analysis (B). ##P < 0.01 compared with CC group.
Figure 5
Figure 5
The effect of IL-1β-511 WT and SNP on Bax in MI-induced cell. Bax protein expression using Western blotting assays (A) and Bax protein expression using Statistical Analysis (B). ##P < 0.01 compared with CC group.
Figure 6
Figure 6
IL-1β-511 WT and SNP and Risk of Ischemic Stroke at Young Age. CC, IL-1β-511CC (WT/WT); CT, IL-1β-511 CT (WT/SNP); TT, IL-1β-511 TT (SNP/SNP). ##P < 0.01 compared with CC group; #P < 0.01 compared with CT group.
Figure 7
Figure 7
The effect of IL-1β-511 WT and SNP on NF-κB in Stroke-induced cell. NF-κB activity (A), NF-κB protein expression using Western blotting assays (B) and NF-κB protein expression using Statistical Analysis (C). ##P < 0.01 compared with CC group.
Figure 8
Figure 8
The effect of IL-1β-511 WT and SNP on iNOS in Stroke-induced cell. iNOS activity (A), iNOS protein expression using Western blotting assays (B) and iNOS protein expression using Statistical Analysis (C). ##P < 0.01 compared with CC group.
Figure 9
Figure 9
The effect of IL-1β-511 WT and SNP on MMP-2 in Stroke-induced cell. MMP-2 protein expression using Western blotting assays (A) and iNOS protein expression using Statistical Analysis (B). ##P < 0.01 compared with CC group.
Figure 10
Figure 10
The effect of IL-1β-511 WT and SNP on Bax in Stroke-induced cell. Bax protein expression using Western blotting assays (A) and Bax protein expression using Statistical Analysis (B). ##P < 0.01 compared with CC group.
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