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. 2015 Oct;35(5):321-4.
doi: 10.14639/0392-100X-588.

Macrophageal infiltration and microvessel density in laryngeal carcinoma: study of 52 cases

T M Popov  1 , T Dikov  2 , T E Goranova  3 , G Stancheva  3 , R P Kaneva  3 , S Todorov  1 , O Stoyanov  1 , J Rangachev  1 , T Marinov  1 , D P Popova  1 , V I Mitev  3 , D Konov  1
Affiliations

Macrophageal infiltration and microvessel density in laryngeal carcinoma: study of 52 cases

T M Popov et al. Acta Otorhinolaryngol Ital. 2015 Oct.

Abstract
in English, Italian

Angiogenesis is one of the six originally constituted hallmarks of cancer that has been extensively studied in the last two decades. The aim of our study is to assess the microvessel and macrophageal density in laryngeal carcinoma and its clinicopathological correlations. We immunohistochemically assessed microvessel density (CD34) and macrophage count (CD68) using microarray techniques and then looked for clinicopathological correlations. The mean micro-vessel density in the study group was 14.27 ± 12.92 vessels in a ×200 field with a mean macrophageal infiltration density of 5.19 ± 4.32. Median microvessel density was significantly higher in patients with metastasis than in patients without metastasis. Additionally, linear regression established that macrophageal infiltration density could predict microvessel density in laryngeal carcinoma. We found no association between either factor and recurrence rate or other clinical characteristics. Our study adds additional data to a problem that has been widely studied during the last two decades, even if controversies in this area still remain.

L'angiogenesi è uno dei sei principali meccanismi alla base del cancro, ed è stato studiato approfonditamente negli ultimi 20 anni. L'obiettivo del presente studio è stato quello di determinare sia la densità capillare sia l'infiltrato macrofagico nei campioni di carcinoma laringeo e di determinarne la correlazione con gli aspetti clinici e patologici. Sia la densità capillare (CD34) sia l'infiltrato macrofagico (CD68) sono stati determinati con metodiche immunoistochimiche mediante microarray. Il nostro campione ha mostrato una densità capillare media di 14,27 ± 12,92 vasi su campo ingrandito a 200×, e l'infiltrato macrofagico medio è stato di 5,19 ± 4,32. La densità capillare si è dimostrata superiore nei pazienti metastatici. Inoltre uno studio di regressione lineare ha mostrato che l'entità dell'infiltrato macrofagico poteva predire la densità capillare del campione di carcinoma laringeo preso in esame. Non abbiamo invece individuato una correlazione fra ambo i fattori studiati e l'incidenza delle recidive o gli altri fattori clinici presi in esame. Il nostro studio aggiunge dati ad un problema che per quanto studiato a fondo negli ultimi 20 anni resta nella sostanza controverso.

Keywords: CD31; CD68; Laryngeal carcinoma; Macrophage count; Micro-vessel density.

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Figures

Fig. 1.
Fig. 1.
CD31+ immunohistochemical visualisation of microvessel density in laryngeal carcinoma (A, B). CD68+ immunohistochemical visualisation of macrophageal infiltration density in laryngeal carcinoma (C, D).
Fig. 2.
Fig. 2.
Microvessel density in patients with and without lymph node metastasis at the time of surgery.
Fig. 3.
Fig. 3.
Linear correlation between microvessel and macrophageal infiltration density in laryngeal carcinoma.
See this image and copyright information in PMC

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