VEGF Gene Polymorphisms Affect Serum Protein Levels and Alter Disease Activity and Synovial Lesions in Rheumatoid Arthritis

Abstract

Background: Our study investigated 2 common single-nucleotide polymorphisms (SNPs) of vascular endothelial growth factor (VEGF) for their influences on serum VEGF levels, disease activity, and synovial lesions in rheumatoid arthritis (RA).

Material/methods: Clinical information and venous blood samples were collected from 98 RA patients and 100 healthy controls. Genotyping on samples from the subjects was performed using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Serum VEGF levels were determined using the enzyme-linked immunosorbent assay (ELISA). The synovial thickness and joint effusion of 28 joints were measured in RA patients, and total sharp score (TSS) and disease activity score (DAS) of 28 joints were recorded.

Results: The genotype and allele frequencies of VEGF rs833070 (G>A) and rs3025030 (G>C) were significantly different between RA group and control group (all P<0.05). VEGF rs833070 and rs3025030 polymorphisms were associated with increasing VEGF serum levels in the RA group (all P<0.01). Statistically significant difference was observed in DAS28 between the different genotypes of VEGF rs833070 in RA patients (P<0.05). Importantly, significant differences in synovial thickening, joint effusion and synovial angiogenesis were observed between the different genotypes of VEGF rs833070 and rs3025030 polymorphisms (all P<0.05).

Conclusions: Our study provides evidence that VEGF polymorphisms might be important indicators of disease activity and synovial lesions, and prognostic factors in evaluating the treatment effectiveness in RA.

Figure 1

Comparison of serum vascular endothelial growth factor (VEGF) levels between GG, AG, and AA genotype of VEGF rs833070 polymorphisms in rheumatoid arthritis patients. The serum levels of VEGF were determined in 98 rheumatoid arthritis patients. ^* Compared with AA genotype, P<0.01.

Figure 2

Comparison of serum vascular endothelial growth factor (VEGF) levels between CC, GC, and GG genotype of VEGF rs3025030 polymorphisms in rheumatoid arthritis patients. The serum levels of VEGF were determined in 98 rheumatoid arthritis patients. ^* Compared with CC genotype, P<0.01.