Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Oct;86(20):7961-5.
doi: 10.1073/pnas.86.20.7961.

Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli

J Chen  1 , B Derfler, A Maskati, L Samson
Affiliations

Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli

J Chen et al. Proc Natl Acad Sci U S A. 1989 Oct.

Abstract

If eukaryotic genes could protect bacteria with defects in DNA repair, this effect could be exploited for the isolation of eukaryotic DNA repair genes. We have thus cloned a DNA repair gene from Saccharomyces cerevisiae that directs the synthesis of a DNA glycosylase that specifically releases 3-methyladenine from alkylated DNA and in so doing protects alkylation-sensitive Escherichia coli from killing by methylating agents. The cloned yeast gene was then used to generate a mutant strain of S. cerevisiae that carries a defect in the glycosylase gene and is extremely sensitive to DNA methylation. This approach may allow the isolation of a large number of eukaryotic DNA repair genes.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4281-5 - PubMed
    1. Nature. 1982 Apr 22;296(5859):773-5 - PubMed
    1. Mol Gen Genet. 1984;197(3):368-72 - PubMed
    1. J Biol Chem. 1985 Aug 25;260(18):10299-307 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7656-60 - PubMed

Publication types

MeSH terms