Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis
- PMID: 2682657
- PMCID: PMC298356
- DOI: 10.1073/pnas.86.22.8703
Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis
Abstract
Chemotactic behavior in Escherichia coli is mediated by membrane-associated chemoreceptors that transmit sensory signals to the flagellar motors through an intracellular signaling system, which appears to involve a protein phosphorylation cascade. This study concerns the role of CheW, a cytoplasmic protein, in coupling methyl-accepting chemotaxis proteins (MCPs), the major class of membrane receptors, to the intracellular signaling system. Steady-state flagellar rotation behavior was examined in a series of strains with different combinations and relative amounts of CheW, MCPs, and other signaling components. At normal expression levels, CheW stimulated clockwise rotation, and receptors appeared to enhance this stimulatory effect. At high expression levels, MCPs inhibited clockwise rotation, and CheW appeared to augment this inhibitory effect. Since overexpression of CheW or MCP molecules had the same behavioral effect as their absence, chemoreceptors probably use CheW to modulate two distinct signals, one that stimulates and one that inhibits the intracellular phosphorylation cascade.
Similar articles
-
Bacterial chemotaxis coupling protein: Structure, function and diversity.Microbiol Res. 2019 Feb;219:40-48. doi: 10.1016/j.micres.2018.11.001. Epub 2018 Nov 6. Microbiol Res. 2019. PMID: 30642465 Review.
-
Methylation segments are not required for chemotactic signalling by cytoplasmic fragments of Tsr, the methyl-accepting serine chemoreceptor of Escherichia coli.Mol Microbiol. 1996 Feb;19(4):737-46. doi: 10.1046/j.1365-2958.1996.408930.x. Mol Microbiol. 1996. PMID: 8820644
-
Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli.J Bacteriol. 1989 Sep;171(9):5190-3. doi: 10.1128/jb.171.9.5190-5193.1989. J Bacteriol. 1989. PMID: 2670907 Free PMC article.
-
Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY.Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6269-73. doi: 10.1073/pnas.88.14.6269. Proc Natl Acad Sci U S A. 1991. PMID: 2068106 Free PMC article.
-
Bacterial chemotaxis: biochemistry of behavior in a single cell.Crit Rev Microbiol. 1985;12(2):95-130. doi: 10.3109/10408418509104426. Crit Rev Microbiol. 1985. PMID: 2992881 Review.
Cited by
-
Mutational analysis of the control cable that mediates transmembrane signaling in the Escherichia coli serine chemoreceptor.J Bacteriol. 2011 Oct;193(19):5062-72. doi: 10.1128/JB.05683-11. Epub 2011 Jul 29. J Bacteriol. 2011. PMID: 21803986 Free PMC article.
-
Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.Proc Natl Acad Sci U S A. 1990 Jan;87(1):41-5. doi: 10.1073/pnas.87.1.41. Proc Natl Acad Sci U S A. 1990. PMID: 2404281 Free PMC article.
-
Polar localization of a soluble methyl-accepting protein of Pseudomonas aeruginosa.J Bacteriol. 2005 Nov;187(22):7840-4. doi: 10.1128/JB.187.22.7840-7844.2005. J Bacteriol. 2005. PMID: 16267307 Free PMC article.
-
Motility and chemotaxis of filamentous cells of Escherichia coli.J Bacteriol. 2000 Aug;182(15):4337-42. doi: 10.1128/JB.182.15.4337-4342.2000. J Bacteriol. 2000. PMID: 10894745 Free PMC article.
-
Computer simulation of the phosphorylation cascade controlling bacterial chemotaxis.Mol Biol Cell. 1993 May;4(5):469-82. doi: 10.1091/mbc.4.5.469. Mol Biol Cell. 1993. PMID: 8334303 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous