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. 2016 Jan 5;3(1):e000148.
doi: 10.1136/vetreco-2015-000148. eCollection 2016.

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay

S J Wynwood  1 , M A Burns  2 , G C Graham  3 , S L Weier  4 , D B McKay  5 , S B Craig  6
Affiliations

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay

S J Wynwood et al. Vet Rec Open. .

Erratum in

  • Vet Rec Open. 2016;3: doi: 10.1136/vetreco-2015-000148corr1

Abstract

Leptospirosis causes significant economic loss within the cattle industry worldwide. Current diagnostic methods are generally inadequate for dealing with large numbers of samples, are outdated, and provide little useful diagnostic and epidemiological information. This aim of this study was to apply a microsphere immunoassay (MIA), utilising Luminex xMap technology, to 200 bovine serum samples to determine this method's usefulness in leptospirosis diagnosis in comparison with the current gold standard, the microscopic agglutination test (MAT). Although MAT is the most widely used laboratory test for the diagnosis of leptospirosis, its reliance on live cultures, subjective interpretation of results and an inability to differentiate between antibody classes, suggest MAT is no longer the best method for the diagnosis of leptospirosis. The results presented in this paper show that MIA was able to determine reactive from non-reactive samples when compared with MAT, and was able to differentiate IgG and IgM classes of antibody. The results suggest increased sensitivity in MIA and the ability to multiplex up to 500 antigens at one time allows for significant improvements in cost-effectiveness as well as a reduced dependency on live cultures. The relatively low cost, high throughput platform and differentiation of antibody class, as shown in previous research, make this assay worthy of consideration for the diagnosis of leptospirosis in small-scale or large-scale bovine populations.

Keywords: Cattle; Dairy cattle; Diagnostics; Leptospira; Leptospira hardjo.

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Figures

FIG 1
FIG 1
A comparison of the microsphere immunoassay (MIA) and microscopic agglutination test (MAT) reactive samples
FIG 2
FIG 2
Analysis of reactive samples by antibody type measured by microsphere immunoassay (MIA)
FIG 3
FIG 3
Serovar analysis of a typical Hardjo reactive bovine sample. MAT, microscopic agglutination test; MIA, microsphere immunoassay
FIG 4
FIG 4
Non-specific cross-reacting IgM sample
See this image and copyright information in PMC

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