In vitro evaluation of the risk of inflammatory response after chitosan/HA and chitosan/β-1,3-glucan/HA bone scaffold implantation

Abstract

The aim of the study was to evaluate in vitro the risk of inflammatory response induced by chitosan/hydroxyapatite (chit/HA) and novel chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) bone scaffolds. The inflammatory response was assessed via measurement of proinflammatory cytokine and ROI production by human monocytes, macrophages, and osteoblasts stimulated with investigated scaffolds. Moreover, adsorption of human serum/plasma proteins to the tested materials was determined. Both biomaterials did not induce intracellular ROI generation by monocytes, macrophages, and osteoblasts and did not stimulate proinflammatory cytokine (IL-6 and TNF-α) production by inflammatory cells. Moreover, the chit/glu/HA material induced increased TNF-α production by osteoblasts that is believed to enhance osteogenic differentiation. Thus, it was demonstrated that chit/HA and chit/glu/HA scaffolds carry a low risk of biomaterial-induced inflammatory response and are promising materials as bone scaffolds for bone tissue engineering and regenerative medicine applications.

Keywords: Biomaterial-induced inflammation; Bone tissue engineering; Fibrinogen; Protein adsorption; Reactive oxygen intermediates; Tumour necrosis factor-alpha.