Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
- PMID: 26840225
- DOI: 10.1002/9780470151808.sc01g03s36
Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
Abstract
Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.
Keywords: FGF; Hhex; activin; anterior; differentiation; embryonic stem cells; endoderm.
Copyright © 2016 John Wiley & Sons, Inc.
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