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Review
. 2016 Feb 3:36:1G.3.1-1G.3.12.
doi: 10.1002/9780470151808.sc01g03s36.

Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

Michaela Rothová  1 , Jurriaan J Hölzenspies  1 , Alessandra Livigni  2 , Santiago Nahuel Villegas  2   3 , Joshua M Brickman  1   2
Affiliations
Review

Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors

Michaela Rothová et al. Curr Protoc Stem Cell Biol. .

Abstract

Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.

Keywords: FGF; Hhex; activin; anterior; differentiation; embryonic stem cells; endoderm.

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References

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