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. 2016 Apr;54(4):1144-7.
doi: 10.1128/JCM.02760-15. Epub 2016 Feb 3.

Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria

Belén Rodríguez-Sánchez  1 , M Jesús Ruiz-Serrano  2 , Adrián Ruiz  3 , Markus Timke  4 , Markus Kostrzewa  4 , Emilio Bouza  5
Affiliations

Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria

Belén Rodríguez-Sánchez et al. J Clin Microbiol. 2016 Apr.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has demonstrated its ability to promptly identify nontuberculous mycobacteria using the Mycobacteria Library v2.0. However, some species are particularly difficult to identify reliably using this database, providing a low log(score). In this study, the identification power of an updated Mycobacteria Library (v3.0) has been evaluated. Overall, 109 NTM isolates were analyzed with both databases. The v3.0 database allowed a high-level confidence in the identification [log(score) value, ≥1.8] of 91.7% of the isolates versus 83.5% with the v2.0 version (P< 0.01).

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