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. 2016 Jun;249(3):375-80.
doi: 10.1007/s00232-016-9875-4. Epub 2016 Feb 3.

Equilibria Between Cell Membranes and Electrolyte Solution: Effect of Fatal Accidental Hypothermia

Aneta D Petelska  1 , Joanna Kotyńska  2 , Monika Naumowicz  2 , Zbigniew A Figaszewski  2
Affiliations

Equilibria Between Cell Membranes and Electrolyte Solution: Effect of Fatal Accidental Hypothermia

Aneta D Petelska et al. J Membr Biol. 2016 Jun.

Abstract

Equilibria between the membranes of erythrocytes as well as thrombocytes and solution ions in fatal accidental hypothermia were analyzed using a theoretical four-equilibria model. The model was developed to determinate parameters characterizing cell membrane-surrounding ion interactions: the total surface concentrations of both acidic and basic groups C A, C B, and association constants K AH, K BOH. Knowledge of these parameters was necessary to calculate the theoretical values of surface charge density. The model was validated by curve-fitting the experimental data points to simulated data generated by the model. The experimental and theoretical surface charge density values agree at pH 2-8, at higher pH the deviation was observed.

Keywords: Acid–base equilibria; Erythrocytes; Fatal accidental hypothermia; Microelectrophoresis; Surface charge density; Thrombocytes.

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Figures

Fig. 1
Fig. 1
Surface charge density of erythrocytes membrane as a function of the pH of the electrolyte solution: times—control, black circle—sudden unexpected death, white square—fatal accidental hypothermia. Points denote experimental values. Continuous line links the theoretical values
Fig. 2
Fig. 2
Surface charge density of thrombocytes membrane as a function of the pH of the electrolyte solution: times—control, black circle—sudden unexpected death, white square—fatal accidental hypothermia. Points denote experimental values. Continuous line links the theoretical values
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