doi: 10.1007/978-1-4939-3393-8_9.
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Affiliations
- PMID: 26843168
- DOI: 10.1007/978-1-4939-3393-8_9
Item in Clipboard
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Methods Mol Biol.
2016.
Abstract
Short peptide tags genetically fused to recombinant proteins have been widely used to facilitate detection or purification without the need to develop specific procedures. In general, an ideal affinity tag would allow the efficient purification of tagged proteins in high yield, without affecting its function. Here, we describe the purification steps to purify a recombinant polyhistidine-tagged glucosyltransferase from Centella asiatica using immobilized metal affinity chromatography.
Keywords: Glucosyltransferase; His-tagged protein; IMAC; Purification; Recombinant.
Similar articles
-
Purification of Polyhistidine-Tagged Proteins.Methods Mol Biol. 2017;1485:275-303. doi: 10.1007/978-1-4939-6412-3_14. Methods Mol Biol. 2017. PMID: 27730558
-
Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.Biotechnol Bioeng. 2005 Nov 20;92(4):501-13. doi: 10.1002/bit.20633. Biotechnol Bioeng. 2005. PMID: 16080185
-
Purification of poly-histidine-tagged proteins.Methods Mol Biol. 2011;681:311-35. doi: 10.1007/978-1-60761-913-0_17. Methods Mol Biol. 2011. PMID: 20978973
-
Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions.Biotechnol Lett. 2011 Jun;33(6):1075-84. doi: 10.1007/s10529-011-0554-3. Epub 2011 Feb 12. Biotechnol Lett. 2011. PMID: 21318632 Review.
-
Immobilized-metal affinity chromatography (IMAC): a review.Methods Enzymol. 2009;463:439-73. doi: 10.1016/S0076-6879(09)63027-5. Methods Enzymol. 2009. PMID: 19892187 Review.
Cited by
-
DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, Burkholderia dolosa PC543: effect of N-/C-terminal His Tag extension on protein solubility and activity.Eng Life Sci. 2018 Oct 31;18(12):893-903. doi: 10.1002/elsc.201800036. eCollection 2018 Dec. Eng Life Sci. 2018. PMID: 32624883 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
