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. 2016 Feb 5:21:4.
doi: 10.1186/s40001-016-0199-8.

Cytokine expression in the visceral adipose tissue after laparoscopic and conventional surgery in a rodent model

Philipp Lingohr  1 , Jonas Dohmen  2 , Hanno Matthaei  3 , Nils Konieczny  4 , Juliane Hoffmann  5 , Edwin Bölke  6 , Sven Wehner  7 , Jörg C Kalff  8
Affiliations

Cytokine expression in the visceral adipose tissue after laparoscopic and conventional surgery in a rodent model

Philipp Lingohr et al. Eur J Med Res. .

Abstract

Background: Laparoscopic Surgery has become a worldwide standard procedure for a variety of indications. This has been attributed to a milder postoperative inflammatory response by the innate immune system potentially mediated through immune mediators released by the visceral adipose tissue (VAT). However, an in vivo experimental evidence is lacking and is the issue of our present study.

Methods: Male Wistar rats (N = 24) underwent standardized surgical procedures of conventional cecum resection (CCR), conventional sham operation, laparoscopic cecum resection (LCR), or laparoscopic sham operation. Cytokine expression of leptin, resistin, and IL-6 was analyzed in VAT before and after resection by quantitative RT-PCR.

Results: Postoperative leptin gene expression was reduced in the CCR and LCR groups, while expression was not significantly affected in both sham groups compared to the preoperative levels. In contrast, IL-6 expression was not affected in the LCR group, but was significantly elevated in the CCR cohort. The IL-6 expression was significantly higher in CCR compared to LCR. Resistin expression levels did not differ between all groups.

Conclusions: Our study underlines the role of immunological involvement of VAT in the postoperative phase. Low leptin levels seem to act as a stimulator for energy uptake in order to cope with postoperative stress. A lower IL-6 expression in the LCR compared to the CCR group may indicate a weaker inflammatory activity potentially adding to the clinical benefits observed in patients undergoing LS.

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Figures

Fig. 1
Fig. 1
Timeline showing the experimental process
Fig. 2
Fig. 2
Leptin expression. Pre- and postoperative leptin expression in VAT with a significantly decreased postoperative expression in the CCR (*p < 0.05) and LCR (**p < 0.01) group
Fig. 3
Fig. 3
IL-6 expression. Pre- and postoperative IL-6 expression in VAT with a significantly increased postoperative expression in the CCR group (**p < 0.01) also compared to the LCR group (*p < 0.05)
Fig. 4
Fig. 4
Resistin expression. Pre- and postoperative resistin expression in VAT with no significant changes in any group
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