doi: 10.1016/0378-1119(89)90032-2.
The catalytic core of the sunY intron of bacteriophage T4
Affiliations
- PMID: 2684777
- DOI: 10.1016/0378-1119(89)90032-2
Item in Clipboard
The catalytic core of the sunY intron of bacteriophage T4
Gene.
.
Abstract
The self-splicing sunY intron of bacteriophage T4 shares a common secondary structure with other group I introns. A long open reading frame within the intron is entirely 3' of the structural elements conserved in all group I introns. This catalytic core is the smallest yet described for a self-splicing intron. An internal deletion of 728 nucleotides (nt), leaving 196 nt at the 5' end and 109 nt at the 3' end, allows normal self-splicing. Transcripts terminating 196 nt 3' of the 5' splice site retain catalytic activity.
Similar articles
-
Miniribozymes, small derivatives of the sunY intron, are catalytically active.Mol Cell Biol. 1989 Dec;9(12):5480-3. doi: 10.1128/mcb.9.12.5480-5483.1989. Mol Cell Biol. 1989. PMID: 2685567 Free PMC article.
-
Mechanism of 3' splice site selection by the catalytic core of the sunY intron of bacteriophage T4: the role of a novel base-pairing interaction in group I introns.Genes Dev. 1990 May;4(5):777-88. doi: 10.1101/gad.4.5.777. Genes Dev. 1990. PMID: 2379829
-
Spontaneous shuffling of domains between introns of phage T4.Nature. 1990 Jul 26;346(6282):394-6. doi: 10.1038/346394a0. Nature. 1990. PMID: 2197562
-
Intron-associated splicing reactions in bacteriophage T4.Mol Microbiol. 1990 Jun;4(6):867-71. doi: 10.1111/j.1365-2958.1990.tb00659.x. Mol Microbiol. 1990. PMID: 2215214 Review.
-
Phage T4 introns: self-splicing and mobility.Annu Rev Genet. 1990;24:363-85. doi: 10.1146/annurev.ge.24.120190.002051. Annu Rev Genet. 1990. PMID: 2088173 Review. No abstract available.
Cited by
-
Requirements for self-splicing of a group I intron from Physarum polycephalum.Nucleic Acids Res. 1994 Oct 11;22(20):4315-20. doi: 10.1093/nar/22.20.4315. Nucleic Acids Res. 1994. PMID: 7937160 Free PMC article.
-
Widespread distribution of a group I intron and its three deletion derivatives in the lysin gene of Streptococcus thermophilus bacteriophages.J Virol. 2000 Jan;74(2):611-8. doi: 10.1128/jvi.74.2.611-618.2000. J Virol. 2000. PMID: 10623722 Free PMC article.
-
Reconstitution of a group I intron self-splicing reaction with an activator RNA.Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):184-8. doi: 10.1073/pnas.88.1.184. Proc Natl Acad Sci U S A. 1991. PMID: 1986364 Free PMC article.
-
Folding of group I introns from bacteriophage T4 involves internalization of the catalytic core.Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11105-9. doi: 10.1073/pnas.88.24.11105. Proc Natl Acad Sci U S A. 1991. PMID: 1763026 Free PMC article.
-
Evolution of mobile group I introns: recognition of intron sequences by an intron-encoded endonuclease.Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11983-7. doi: 10.1073/pnas.91.25.11983. Proc Natl Acad Sci U S A. 1994. PMID: 7991569 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
