Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging

Abstract

I describe the construction of a variety of Escherichia coli recA deletion strains designed to facilitate molecular cloning. These recA deletion strains permit the efficient cloning of foreign inserts carried in plasmid, phage, cosmid, phasmid (phage-plasmid hybrid) or phosmid (phage-cosmid hybrid) vectors.