Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2016;11(4):e1139277.
doi: 10.1080/15592324.2016.1139277.

Regulation of Arabidopsis MAPKKK18 by ABI1 and SnRK2, components of the ABA signaling pathway

Małgorzata Tajdel  1 , Filip Mituła  1 , Agnieszka Ludwików  1
Affiliations
Review

Regulation of Arabidopsis MAPKKK18 by ABI1 and SnRK2, components of the ABA signaling pathway

Małgorzata Tajdel et al. Plant Signal Behav. 2016.

Abstract

The plant hormone abscisic acid (ABA), a key regulator in many crucial developmental and physiological processes, recruits diverse components into precisely regulated signaling network. We recently discovered that MAPKKK18, an ABA-activated kinase, is regulated by the protein phosphatase type 2C (PP2C) ABI1 and the kinase SnRK2.6, both components of the ABA core signaling pathway. ABI1 acts to inhibit MAPKKK18 kinase activity, but also affects MAPKKK18 protein turnover via the ubiquitin-proteasome pathway. SnRK2.6 kinase also seems to be important for the regulation of MAPKKK18 function. In this review we summarize the mechanisms that are exclusively involved in MAPKKK18 kinase regulation and that ensure specificity in its activation.

Keywords: ABA signaling; ABI1; Arabidopsis thaliana; MAP kinase cascade; MAPKKK18; PP2C; SnRK2; UPS.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
MAPKKK18 interacts with the SnRK2.6 kinase. (A) Pull-down assay to verify the interaction beteween SnRK2.6 and MAPKKK18. Input lines represent total SnRK2.6. Recombinant His- MAPKKK18, pre-coupled to Ni-NTA agarose, was incubated with recombinant GST-SnRK2.6. GST- and His-tagged proteins were detected (IB) using anti-GST and anti-His tag antibodies, respectively. (B) SnRK2.6 and MAPKKK18 proteins interact within the nucleus. Cytoplasmic interaction around the nucleus is present in about 10% of protoplasts. BiFC analysis in Arabidopsis protoplasts transiently expressing full-length SnRK2.6 and MAPKKK18 fused to cECFP or nVenus, respectively. RFPtag-CBP20 was used as both transformation control and marker of nuclear localization. Scale bar: 20 μm.
See this image and copyright information in PMC

References

    1. Meyer K, Leube MP, Grill E. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science 1994; 264(5164):1452-5; PMID:8197457; http://dx.doi.org/10.1126/science.8197457 - DOI - PubMed
    1. Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A. Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 2009; 324:1064-8; PMID:19407143; http://dx.doi.org/10.1126/science.1172408 - PubMed
    1. Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodriguez A, Chow Tsz-fung, et al. . Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 2009; 324(5930):1068-71; PMID:19407142; http://dx.doi.org/10.1126/science.1173041 - PMC - PubMed
    1. Nishimura N, Sarkeshik A, Nito K, Park SY, Wang A, Carvalho PC, Lee S, Caddell D F, Cutler SR, Chory J, et al. . PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis. Plant J 2010; 61:290-9; PMID:19874541; http://dx.doi.org/10.1111/j.1365-313X.2009.04054.x - DOI - PMC - PubMed
    1. Gosti F, Beaudoin N, Serizet C, Webb A, Vartanian N, Giraudat J. ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 1999; 11:1897-910; PMID:10521520; http://dx.doi.org/10.1105/tpc.11.10.1897 - DOI - PMC - PubMed

Publication types

MeSH terms