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. 2016 Feb 8:40:10F.2.1-10F.2.18.
doi: 10.1002/9780471729259.mc10f02s40.

Genetic Manipulation of Nocardia Species

Dipesh Dhakal  1 , Amit Kumar Jha  1 , Anaya Pokhrel  1 , Anil Shrestha  1 , Jae Kyung Sohng  1
Affiliations

Genetic Manipulation of Nocardia Species

Dipesh Dhakal et al. Curr Protoc Microbiol. .

Abstract

Nocardia spp. are aerobic, Gram-positive, catalase positive, and non-motile actinomycetes. They are associated with human infections. However, some species produce important natural products, degrade toxic chemicals, and are involved in biotransformation of valuable products. The lack of robust genetic tools has hindered detailed studies and advanced research. This unit describes the major genetic engineering approaches using Nocardia sp. CS682 as a prototype. These methods will certainly help in understanding the basis of their pathogenicity as well as biosynthetic and biotransforming abilities. It can be expected that knowledge of the biochemistry behind their pathogenicity will be crucial in developing effective treatment strategies. These genetic tools can be utilized to develop rational metabolic engineering approaches for crafting host strains with higher production or biotransformation ability.

Keywords: Nocardia spp; RNA isolation; genomic DNA; protein extraction; transformation.

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