Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1989 Jul-Aug;23(4):917-23.

[Isolation of individual chromosomes and their use in studying the human and animal genome]

[Article in Russian]
A I Poletaev
  • PMID: 2685546
Review

[Isolation of individual chromosomes and their use in studying the human and animal genome]

[Article in Russian]
A I Poletaev. Mol Biol (Mosk). 1989 Jul-Aug.

Abstract

This mini-review considers the main achievements in the field of human and animal chromosome separation: technical problems of flow-karyotyping and flow-sorting, the types of cell lines and chromosome source used, different protocols of chromosome suspension preparation, fluorochromes for chromosome staining, efficiency of chromosome sorting and purity of the fractions. The use of fractions for creating chromosome-specific human gene libraries and mapping cloned genes and DNA fragment are also considered.

PubMed Disclaimer