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. 2016 Mar;32(1):114-9.
doi: 10.1007/s12288-015-0504-7. Epub 2015 Jan 23.

Fetal Hemoglobin in Tunisian Sickle Cell Disease Patient: Relationship with Polymorphic Sequences Cis to the β-Globin Gene

Imen Moumni  1 , Maha Ben Mustapha  1 , Ikbel Ben Mansour  1 , Amine Zoraï  1 , Kaïs Douzi  1 , Sarah Sassi  1 , Dorra Chaouachi  1 , Fethi Mellouli  2 , Mohamed Bejaoui  2 , Salem Abbes  1
Affiliations

Fetal Hemoglobin in Tunisian Sickle Cell Disease Patient: Relationship with Polymorphic Sequences Cis to the β-Globin Gene

Imen Moumni et al. Indian J Hematol Blood Transfus. 2016 Mar.

Abstract

Fetal hemoglobin (HbF) plays a dominant role in ameliorating morbidity and mortality of hemoglobinopathies. We evaluated the effects of polymorphic markers within the β-globin gene cluster to identify the genetic mechanics that influence HbF on Tunisian sickling patients (n = 242). Haplotype analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the framework polymorphism was established by PCR-sequencing, four independent regions of interest were identified: the 5' region of β-LCR-HS2 site, the intervening sequence II (IVSII) region of two fetal (Gγ and Aγ) genes and the 5' region of β-globin gene. The correlation of these various Haplotypes and SNPs with HbF expression and clinical data was studied. Our data showed that among the various polymorphic markers analyzed, only the sequence (AT)xN12(AT)y in LCR HS2 region was significantly associated (p < 0.05) with increased HbF levels, suggesting that the β-globin gene cluster exerts a significant effect on HbF in sickle cell patients. This study can improve understanding of the physiopathology of the disease and aid to increase our ability to predict clinical severity.

Keywords: Fetal hemoglobin (HbF); Microsatellite repeats; Sickle cell anemia.

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Figures

Fig. 1
Fig. 1
Map of the β-globin gene cluster and the polymorphic
See this image and copyright information in PMC

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