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. 1989 Oct;9(10):4488-94.
doi: 10.1128/mcb.9.10.4488-4494.1989.

Time of replication of ARS elements along yeast chromosome III

A E Reynolds  1 , R M McCarroll, C S Newlon, W L Fangman
Affiliations

Time of replication of ARS elements along yeast chromosome III

A E Reynolds et al. Mol Cell Biol. 1989 Oct.

Abstract

The replication of putative replication origins (ARS elements) was examined for 200 kilobases of chromosome III of Saccharomyces cerevisiae. By using synchronous cultures and transfers from dense to light isotope medium, the temporal pattern of mitotic DNA replication of eight fragments that contain ARSs was determined. ARS elements near the telomeres replicated late in S phase, while internal ARS elements replicated in the first half of S phase. The results suggest that some ARS elements in the chromosome may be inactive as replication origins. The actively expressed mating type locus, MAT, replicated early in S phase, while the silent cassettes, HML and HMR, replicated late. Unexpectedly, chromosome III sequences were found to replicate late in G1 at the arrest induced by the temperature-sensitive cdc7 allele.

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References

    1. Mol Cell Biol. 1988 Jun;8(6):2342-9 - PubMed
    1. EMBO J. 1987 Feb;6(2):461-7 - PubMed
    1. Exp Cell Res. 1958 Oct;15(2):350-7 - PubMed
    1. Biochim Biophys Acta. 1969 Jan 21;174(1):246-52 - PubMed
    1. Biochim Biophys Acta. 1971 Oct 14;247(2):310-21 - PubMed

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