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. 2015 Dec 5:6:94-100.
doi: 10.1016/j.dib.2015.11.052. eCollection 2016 Mar.

Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus

J J Cora-Cruz  1 , N Diffoot-Carlo  2 , P A Sundaram  1
Affiliations

Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus

J J Cora-Cruz et al. Data Brief. .

Abstract

Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus.

Keywords: Focal Adhesions; MC3T3-E1 Cells; Mechanical Stimulus; Vinculin.

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Figures

Fig. 1.
Fig. 1
Vinculin expression of MC3T3-E1 cells after cyclic and static loading conditions cultured on PDMS at ratios of 5:1, 10:1 and 15:1 under static conditions (left) and 1% cyclic strain at 0.05 Hz (right). The principal strain axis is parallel to the scale. Each column shows the confocal images of the vinculin expression at a magnification of 60×.
See this image and copyright information in PMC

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