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. 2015 Dec;63(12):888-90.
doi: 10.4103/0301-4738.176040.

Effect of lanosterol on human cataract nucleus

P Mahesh Shanmugam  1 , Aditya Barigali, Jayant Kadaskar, Sandip Borgohain, Divaynsh Kailash Chandra Mishra, Rajesh Ramanjulu, C K Minija
Affiliations

Effect of lanosterol on human cataract nucleus

P Mahesh Shanmugam et al. Indian J Ophthalmol. 2015 Dec.

Abstract

Aim: To study the effect of lanosterol on age-related cataractous human lens nuclei.

Materials and methods: Forty age-related cataractous nuclei removed during manual small incision cataract surgery were obtained and randomly immersed in 25 mM lanosterol solution or in control solution and stored at room temperature for 6 days. Pre- and post-immersion photographs were graded by two masked observers and collated for the regression or progression of lens opacity.

Results: Both lanosterol and control groups showed progression or no change in the lens opacity at the end of 6 days.

Conclusion: Lanosterol 25 mM solution did not reverse opacification of human age-related cataractous nuclei.

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Figures

Figure 1
Figure 1
Lens opacity grading system1: Grade 0: Gridlines clearly seen; (a) Grade 1: Minimal clouding of gridlines, with gridlines still visible; (b) Grade 2: Moderate clouding of gridlines with main gridlines visible; (c) Grade 3: Total clouding of gridlines with gridlines not seen at all
Figure 2
Figure 2
(a and c) Pre incubation images of lens nuclei in lanosterol and control solution respectively. (b and d) Post incubation images of the same nuclei after incubation in Lanosterol and control solution for 6 days; respectively
Figure 3
Figure 3
The comparison of means of control and lanosterol groups; before and after the test, showed no statistically significant difference
Figure 4
Figure 4
(a) Pre- and (b) post-incubation images of the lanosterol incubated nucleus graded as regression of lenticular opacity by one observer
See this image and copyright information in PMC

Comment in

  • Comment to: Effect of lanosterol on human nuclei.
    Venkatesh R, Gurav P, Tibrewal S. Venkatesh R, et al. Indian J Ophthalmol. 2016 Jun;64(6):475. doi: 10.4103/0301-4738.187689. Indian J Ophthalmol. 2016. PMID: 27488164 Free PMC article. No abstract available.
  • Authors' reply.
    Shanmugam PM, Barigali A, Kadaskar J, Borgohain S, Kailash Chandra MD, Rajesh R, Minija CK. Shanmugam PM, et al. Indian J Ophthalmol. 2016 Jun;64(6):476. doi: 10.4103/0301-4738.187691. Indian J Ophthalmol. 2016. PMID: 27488165 Free PMC article. No abstract available.

References

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