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Case Reports
. 1977 Jul;74(7):3047-51.
doi: 10.1073/pnas.74.7.3047.

Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia

G F Temple, J C Chang, Y W Kan
Case Reports

Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia

G F Temple et al. Proc Natl Acad Sci U S A. 1977 Jul.

Abstract

In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.

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References

    1. Br Med Bull. 1976 Sep;32(3):262-9 - PubMed
    1. Eur J Biochem. 1977 Feb;72(3):465-78 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
    1. Nature. 1976 Oct 7;263(5577):471-5 - PubMed
    1. Ann N Y Acad Sci. 1974 Nov 29;241(0):290-309 - PubMed

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