Case Reports
doi: 10.1073/pnas.74.7.3047.
Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia
- PMID: 268654
- PMCID: PMC431405
- DOI: 10.1073/pnas.74.7.3047
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Case Reports
Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia
Proc Natl Acad Sci U S A.
1977 Jul.
Abstract
In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.
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