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Case Reports
. 2016 Feb 9:11:14.
doi: 10.1186/s40793-015-0129-3. eCollection 2016.

Genome sequence of Shinella sp. strain DD12, isolated from homogenized guts of starved Daphnia magna

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Case Reports

Genome sequence of Shinella sp. strain DD12, isolated from homogenized guts of starved Daphnia magna

Anja Poehlein et al. Stand Genomic Sci. .

Abstract

Shinella sp. strain DD12, a novel phosphite assimilating bacterium, has been isolated from homogenized guts of 4 days starved zooplankton Daphnia magna. Here we report the draft genome of this bacterium, which comprises 7,677,812 bp and 7505 predicted protein-coding genes.

Keywords: Alphaproteobacteria; Nitrate reduction; Phosphite assimilation; Shinella.

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Figures

Fig. 1
Fig. 1
Images of Shinella sp. strain DD12 using scanning (left) electron microscopy and the appearance of colony morphology on solid (middle), and liquid (right) nutrient agar medium
Fig. 2
Fig. 2
Phylogenetic tree highlighting the position of Shinella sp. strain DD12, based on 16S rRNA gene sequences
Fig. 3
Fig. 3
Tblastx comparison of the C-P lyase complex. An E-value cutoff of 1e-10 was used and visualization was done with the program Easyfig [55]. Functional genes of the C-P lyase complex were marked in green, accessory genes in orange, the ABC-type transporter in blue and the regulatory subunit in red. Genes not directly associated with this pathway are marked in grey
Fig. 4
Fig. 4
Tblastx comparison of Alphaproteobacteria phnWAY operon. An E-value cutoff of 1e-10 was used and visualization was done with the program Easyfig [55]. The phnWAY cluster was marked in orange tones; sodium/phosphonate symporter (yjbB) was marked in purple; the genes coding ABC-type ferric uptake system (fbpABC) were marked in blue; the transcriptional regulator (lysR) is shown in green

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