Conservation genetics in Chinese sheep: diversity of fourteen indigenous sheep (Ovis aries) using microsatellite markers

Abstract

The domestic sheep (Ovis aries) has been an economically and culturally important farm animal species since its domestication around the world. A wide array of sheep breeds with abundant phenotypic diversity exists including domestication and selection as well as the indigenous breeds may harbor specific features as a result of adaptation to their environment. The objective of this study was to investigate the population structure of indigenous sheep in a large geographic location of the Chinese mainland. Six microsatellites were genotyped for 611 individuals from 14 populations. The mean number of alleles (±SD) ranged from 7.00 ± 3.69 in Gangba sheep to 10.50 ± 4.23 in Tibetan sheep. The observed heterozygote frequency (±SD) within a population ranged from 0.58 ± 0.03 in Gangba sheep to 0.71 ± 0.03 in Zazakh sheep and Minxian black fur sheep. In addition, there was a low pairwise difference among the Minxian black fur sheep, Mongolian sheep, Gansu alpine merino, and Lanzhou fat-tailed sheep. Bayesian analysis with the program STRUCTURE showed support for 3 clusters, revealing a vague genetic clustering pattern with geographic location. The results of the current study inferred high genetic diversity within these native sheep in the Chinese mainland.

Keywords: China; Diversity; indigenous sheep; microsatellite.

Figure 1

Population average pairwise differences of 14 native Chinese sheep. Above diagonal is average number of pairwise differences between populations, Diagonal elements is average number of pairwise differences within population and below diagonal is corrected average pairwise difference.

Figure 2

Clustering diagrams of 14 Chinese sheep populations obtained from K = 2 to K = 14 with best similarities. #label is the most optimal K‐value. Note: number of population: TS (1), ZT (2), AD (3), HZK (4), HU (5), HBR (6), STH (7), TAN (8),GB (9), UQ (10), MXB (11), MGH (12), GSH (13), LZD (14). Superscript letter (A) is L(K), superscript letter (B) is ΔK = m| L″(K)|/s|L(K)|.