Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jan;11(1):717-722.
doi: 10.3892/ol.2015.3912. Epub 2015 Nov 13.

Pantoprazole inhibits human gastric adenocarcinoma SGC-7901 cells by downregulating the expression of pyruvate kinase M2

Yonghua Shen  1 , Min Chen  1 , Shuling Huang  1 , Xiaoping Zou  1
Affiliations

Pantoprazole inhibits human gastric adenocarcinoma SGC-7901 cells by downregulating the expression of pyruvate kinase M2

Yonghua Shen et al. Oncol Lett. 2016 Jan.

Abstract

The Warburg effect is important in tumor growth. The human M2 isoform of pyruvate kinase (PKM2) is a key enzyme that regulates aerobic glycolysis in tumor cells. Recent studies have demonstrated that PKM2 is a potential target for cancer therapy. The present study investigated the effects of pantoprazole (PPZ) treatment and PKM2 transfection on human gastric adenocarcinoma SGC-7901 cells in vitro. The present study revealed that PPZ inhibited the proliferation of tumor cells, induced apoptosis and downregulated the expression of PKM2, which contributes to the current understanding of the functional association between PPZ and PKM2. In summary, PPZ may suppress tumor growth as a PKM2 protein inhibitor.

Keywords: apoptosis; gastric cancer; proton pump inhibitors; pyruvate kinase M2; tumor metabolism.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Comparison of the cell viability of SGC-7901 subsequent to treatment with 5 mg/ml PPZ and PKM2 transfection for 24 h. The experiment was repeated three times. *P<0.05; **P<0.01. PPZ, pantoprazole; PKM2, M2 isoform of pyruvate kinase; PPZ+, treatment with PPZ; PPZ−, without PPZ treatment; PKM2+, transfection with PKM2; PKM2−, without PKM2 transfection.
Figure 2.
Figure 2.
(A) The apoptosis rate of SGC-7901 cells following 5 mg/ml PPZ administration and PKM2 transfection with Annexin V-fluorescein isothiocyanate apoptosis detection. (Aa) PPZ−/PKM2− group, without PPZ and PKM2 intervention. (Ab) PPZ+/PKM2− group, treatment with PPZ without PKM2 transfection. (Ac) PPZ−/PKM2+ group, transfection with PKM2 without PPZ treatment. (Ad) PPZ+/PKM2+ group, intervention with PPZ and PKM2. (B) Comparison of the apoptosis rate of SGC-7901 cells subsequent to intervention with PPZ and PKM2. The experiment was repeated three times. PPZ, pantoprazole; PKM2, M2 isoform of pyruvate kinase.
Figure 3.
Figure 3.
Effects of 5 mg/ml PPZ treatment and PKM2 transfection on PKM2 expression in SGC-7901 cells. (A) Comparison of the PKM2 expression subsequent to PPZ treatment without PKM2 transfection. (B) Comparison of the PKM2 expression subsequent to PKM2 transfection without PPZ treatment. (C) Comparison of the PKM2 expression with PPZ treatment following PKM2 transfection. (*P<0.05; **P<0.01; ***P<0.001). PPZ, pantoprazole; PKM2, M2 isoform of pyruvate kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Figure 4.
Figure 4.
Effects of 5 mg/ml PPZ treatment and PKM2 transfection on the intracellular distribution of PKM2 in SGC-7901 cells. (A) Intracellular distribution of PKM2 in SGC-7901 cells without PPZ treatment and PKM2 transfection (magnification, ×200). (B) Intracellular distribution of PKM2 in SGC-7901 cells subsequent to PPZ treatment without PKM2 transfection (magnification, ×200). (C) Intracellular distribution of PKM2 in SGC-7901 cells subsequent to PKM2 transfection without PPZ treatment (magnification, ×200). (D) Intracellular distribution of PKM2 in SGC-7901 cells subsequent to PPZ treatment and PKM2 transfection (magnification, ×200). PPZ, pantoprazole; PKM2, M2 isoform of pyruvate kinase.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi: 10.1126/science.123.3191.309. - DOI - PubMed
    1. Simonnet H, Alazard N, Pfeiffer K, Gallou C, Béroud C, Demont J, Bouvier R, Schägger H, Godinot C. Low mitochondrial respiratory chain content correlates with tumor aggressiveness in renal cell carcinoma. Carcinogenesis. 2002;23:759–768. doi: 10.1093/carcin/23.5.759. - DOI - PubMed
    1. Jiang K, He B, Lai L, Chen Q, Liu Y, Guo Q, Wang Q. Cyclosporine A inhibits breast cancer cell growth by downregulating the expression of pyruvate kinase subtype M2. Int J Mol Med. 2012;30:302–308. - PubMed
    1. Furtado CM, Marcondes MC, Sola-Penna M, de Souza ML, Zancan P. Clotrimazole preferentially inhibits human breast cancer cell proliferation, viability and glycolysis. PLoS One. 2012;7:e30462. doi: 10.1371/journal.pone.0030462. - DOI - PMC - PubMed
    1. Kosugi M, Ahmad R, Alam M, Uchida Y, Kufe D. MUC1-C oncoprotein regulates glycolysis and pyruvate kinase M2 activity in cancer cells. PLoS One. 2011;6:e28234. doi: 10.1371/journal.pone.0028234. - DOI - PMC - PubMed

LinkOut - more resources