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Comparative Study
. 1989 Dec;171(12):6771-5.
doi: 10.1128/jb.171.12.6771-6775.1989.

Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene

K Ohmiya  1 , T Kajino, A Kato, S Shimizu
Affiliations
Comparative Study

Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene

K Ohmiya et al. J Bacteriol. 1989 Dec.

Abstract

A chromosomal DNA fragment encoding an endo-1,4-beta-glucanase I (Eg I) gene from Ruminococcus albus cloned and expressed in Escherichia coli with pUC18 was fully sequenced by the dideoxy-chain termination method. The sequence contained a consensus promoter sequence and a structural amino acid sequence. The initial 43 amino acids of the protein were deduced to be a signal sequence, since they are missing in the mature protein (Eg I). High homology was found when the amino acid sequence of the Eg I was compared with that of endoglucanase E from Clostridium thermocellum. Codon usage of the gene was not biased. These results suggested that the properties of the Eg I gene from R. albus was specified from the known beta-glucanase genes of the other organisms.

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