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Randomized Controlled Trial
. 2016 Jul;138(1):286-289.e9.
doi: 10.1016/j.jaci.2015.11.030. Epub 2016 Feb 10.

Vitamin D3 treatment of vitamin D-insufficient asthmatic patients does not alter immune cell function

Brandy Reid  1 , Pierre-Olivier Girodet  2 , Jonathan S Boomer  1 , Azza Abdel-Gadir  3 , Kathy Zheng  4 , Michael E Wechsler  5 , Leonard B Bacharier  6 , Susan J Kunselman  7 , Tonya S King  7 , Elliot Israel  4 , Mario Castro  1 , Manuela Cernadas  4 , Jonathan M Green  8
Affiliations
Randomized Controlled Trial

Vitamin D3 treatment of vitamin D-insufficient asthmatic patients does not alter immune cell function

Brandy Reid et al. J Allergy Clin Immunol. 2016 Jul.
No abstract available

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Figures

Figure 1
Figure 1. Intracellular cytokine analysis of CD4+ T cells
Peripheral blood samples were obtained prior to (V3) and after (V6) 12 weeks of treatment with placebo or vitamin D. Intracellular cytokine staining of CD4+ T cells was performed and the percentage of CD4+ T cells expressing IL-4, IFNγ, IL-10 or IL-17A determined. Shown is the fold change from V3 to V6, calculated as the ratio of the V6 value divided by the V3 value for each sample pair. There was no difference in this ratio between vitamin D- and placebo-treated participants. The Th1:Th2 ratio, calculated by dividing the percentage of CD4/IFNγ positive by the percentage of CD4/IL-4 positive cells, was also not different. Shown are individual data points and lines representing the mean ± standard deviation.
Figure 2
Figure 2. Cytokine secretion by stimulated PBMCs and monocytes
A) PBMCs were isolated from samples collected either before (V3) or after (V6) vitamin D supplementation or placebo, and stimulated with α-CD3 and α-CD28 for 48 hours. Secreted cytokines were analyzed in the culture supernatant by cytokine bead array or ELISA (TGFβ only). The fold change from V3 to V6 was calculated by dividing the value at V6 by the value at V3 for each sample pair. B) CD14+ monocytes were isolated from PBMC from vitamin D-deficient asthmatics before (V3) and after (V6) vitamin D supplementation or placebo and stimulated with LPS for 48 hours. IL-6, IL-10 and IL-12p40 production was determined by ELISA. The fold change from V6 to V3 (V6/V3) for each sample pair was calculated and shown are individual data points and lines representing the mean ± standard deviation.
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References

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