Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Apr 22;62(2):209-12.
doi: 10.1262/jrd.2015-149. Epub 2016 Feb 11.

N, N-Dimethylglycine decreases oxidative stress and improves in vitro development of bovine embryos

Toshikiyo Takahashi  1 , Kouya Sasaki, Tamas Somfai, Takashi Nagai, Noboru Manabe, Keisuke Edashige
Affiliations

N, N-Dimethylglycine decreases oxidative stress and improves in vitro development of bovine embryos

Toshikiyo Takahashi et al. J Reprod Dev. .

Abstract

The antioxidant effect of N, N-dimethylglycine (DMG) on in vitro-produced (IVP) bovine embryos was examined. After in vitro fertilization, presumptive zygotes were cultured with or without 0.1 μM DMG under different oxygen tensions. The percentage of embryos developing to the blastocyst stage was lowest under a 20% oxygen concentration without DMG, and it was significantly increased (P < 0.05) by applying a 5% oxygen concentration. Under the 20% oxygen concentration, supplementation of the medium with DMG significantly improved blastocyst development, which was nearly equal to that achieved under 5% oxygen without DMG. Furthermore, a tendentious increase (P = 0.06) in blastocyst cell numbers was observed when DMG was applied. In the second experiment, addition of H2O2 (0.5 mM) to the culture medium significantly (P < 0.01) reduced the percentage of embryos developing to the blastocyst stage. However, DMG supplementation prevented this reduction. In conclusion, DMG enhanced the invitro development of IVP bovine embryos by acting as an antioxidant.

PubMed Disclaimer

References

    1. Takahashi M. Oxidative stress and redox regulation on in vitro development of mammalian embryos. J Reprod Dev 2012; 58: 1–9. - PubMed
    1. Takahashi M, Keicho K, Takahashi H, Ogawa H, Schultz RM, Okano A. Effect of oxidative stress on development and DNA damage in in-vitro cultured bovine embryos by comet assay. Theriogenology 2000; 54: 137–145. - PubMed
    1. Balasubramanian S, Son WJ, Kumar BM, Ock SA, Yoo JG, Im GS, Choe SY, Rho GJ. Expression pattern of oxygen and stress-responsive gene transcripts at various developmental stages of in vitro and in vivo preimplantation bovine embryos. Theriogenology 2007; 68: 265–275. - PubMed
    1. Kito S, Kaneko Y, Yano H, Tateno S, Ohta Y. Developmental responses of 2-cell embryos to oxygen tension and bovine serum albumin in Wistar rats. Exp Anim 2008; 57: 123–128. - PubMed
    1. Corrêa GA, Rumpf R, Mundim TC, Franco MM, Dode MA. Oxygen tension during in vitro culture of bovine embryos: effect in production and expression of genes related to oxidative stress. Anim Reprod Sci 2008; 104: 132–142. - PubMed