Cellular aspects of proximal tubular phosphate reabsorption

Abstract

In vivo manipulations to alter renal Pi reabsorption and the subsequent isolation of proximal tubular brush border membrane vesicles have greatly increased our knowledge about the regulation of renal Pi reabsorption via the Na+/Pi cotransport system. Only recently, direct biochemical and cell-biological access has become possible by the use of established and primary cell cultures. Based on the results obtained with isolated brush border membranes and cultured cells, a model has been presented, which might serve as a basis for future research of the regulatory control mechanisms of the renal Na+/Pi cotransport. At present, a major drawback is the fact that the molecular identity of the Na+/Pi cotransport system is still unknown. The identification of this transport system would certainly be a great step and would allow to verify or falsify one or the other hypotheses postulated in the past few years for the regulatory control mechanism(s) of the renal Na+/Pi cotransport.