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. 2015;35(6):738-47.
doi: 10.5851/kosfa.2015.35.6.738. Epub 2015 Dec 31.

Identification of Antihypertensive Peptides Derived from Low Molecular Weight Casein Hydrolysates Generated during Fermentation by Bifidobacterium longum KACC 91563

Go Eun Ha  1 , Oun Ki Chang  2 , Su-Mi Jo  1 , Gi-Sung Han  1 , Beom-Young Park  1 , Jun-Sang Ham  1 , Seok-Geun Jeong  1
Affiliations

Identification of Antihypertensive Peptides Derived from Low Molecular Weight Casein Hydrolysates Generated during Fermentation by Bifidobacterium longum KACC 91563

Go Eun Ha et al. Korean J Food Sci Anim Resour. 2015.

Abstract

Angiotensin-converting enzyme (ACE) inhibitory activity was evaluated for the low-molecular-weight fraction (<3 kDa) obtained from milk fermentation by Bifidobacterium longum KACC91563. The ACE inhibitory activity in this fraction was 62.3%. The peptides generated from the <3 kDa fraction were identified by liquid chromatography-electrospray ionization quantitative time-of-flight mass spectrometry analysis. Of the 28 peptides identified, 11 and 16 were identified as β-casein (CN) and αs1-CN, respectively. One peptide was identified as κ-CN. Three peptides, YQEPVLGPVRGPFPIIV, QEPVLGPVRGPFPIIV, and GPVRGPFPIIV, from β-CN corresponded to known antihypertensive peptides. We also found 15 peptides that were identified as potential antihypertensive peptides because they included a known antihypertensive peptide fragment. These peptides were as follows: RELEELNVPGEIVE (f1-14), YQEPVLGPVRGPFP (f193-206), EPVLGPVRGPFPIIV (f195-206), PVLGPVRGPFPIIV (f196-206), VLGPVRGPFPIIV (f197-206), and LGPVRGPFPIIV (f198-206) for β-CN; and APSFSDIPNPIGSENSEKTTMPLW (f176-199), SFSDIPNPIGSENSEKT- TMPLW (f178-199), FSDIPNPIGSENSEKTTMPLW (f179-199), SDIPNPIGSENSEKTTMPLW (f180-199), DIPNPIGSENSEKTTMPLW (f181-199), IPNPIGSENSEKTTMPLW (f182-199), PIGSENSEKTTMPLW (f185-199), IGSENSEKTTMPLW (f186-199), and SENSEKTTMPLW (f188-199) for αs1-CN. From these results, B. longum could be used as a starter culture in combination with other lactic acid bacteria in the dairy industry, and/or these peptides could be used in functional food manufacturing as additives for the development of a product with beneficial effects for human health.

Keywords: B. longum; angiotensin converting enzyme; antihypertensive peptide.

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Figures

Fig. 1.
Fig. 1.. Cleavage sites of peptide bonds on bovine β-, αs1-, and κ-casein hydrolyzed after fermentation with Bifidobacterium longum KACC9156. The dot arrows cleaved peptide bonds in this study. The line arrow cleaved peptide bonds reported by Chang et al. (2013).
See this image and copyright information in PMC

References

    1. Arunachalam K. D. Role of bifidobacteria in nutrition, medicine and technology. Nutr. Res. 1999;19:1559–1597.
    1. Chang O. K., Roux E., Awussi A. A., Miclo L., Jardin J., Jameh N., Dary A., Humbert G., Perrin C. Use of a free form of the Streptococcus thermophilus cell envelope protease PrtS as a tool to produce bioactive peptides. Int. Dairy J. 2014;38:104–115.
    1. Chang O. K., Seol K. H., Jeong S. G., Oh M. H., Park B. Y., Perrin C., Ham J. S. Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom. J. Dairy Sci. 2013;96:5544–5555. - PubMed
    1. Collins M. D., Gibson G. R. Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am. J. Clin. Nutr. 1999;69:1052S–1057S. - PubMed
    1. Cushman D. W., Cheung H. S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 1971;20:1637–1648. - PubMed

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