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Review
. 2016 Mar;10(3):431-42.
doi: 10.1016/j.molonc.2016.01.011. Epub 2016 Feb 5.

Gene expression profiling and DNA methylation analyses of CTCs

Evi S Lianidou  1
Affiliations
Review

Gene expression profiling and DNA methylation analyses of CTCs

Evi S Lianidou. Mol Oncol. 2016 Mar.

Abstract

A variety of molecular assays have been developed for CTCs detection and molecular characterization. Molecular assays are based on the nucleic acid analysis in CTCs and are based on total RNA isolation and subsequent mRNA quantification of specific genes, or isolation of genomic DNA that can be for DNA methylation studies and mutation analysis. This review is mainly focused on gene expression and methylation studies in CTCs in various types of cancer.

Keywords: CTCs; Circulating Tumor Cells; Gene expression; Methylation specific PCR; Molecular assays; Molecular characterization; Multiplex RT-PCR; Quantitative PCR (qPCR); RT-qPCR; Reverse transcription PCR.

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Figures

Figure 1
Figure 1
Overview of molecular methods used for gene expression and methylation analyses of CTCs.
Figure 2
Figure 2
Gene expression and methylation analyses of CTCs in various types of cancer.
See this image and copyright information in PMC

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