The Effect of Pre-sowing Seed Treatment with Metal Nanoparticles on the Formation of the Defensive Reaction of Wheat Seedlings Infected with the Eyespot Causal Agent

Abstract

The paper presents research data of lipid peroxidation and lectin activity in wheat seedlings at seed treatment with solution of metal nanoparticles (Zn, Ag, Fe, Mn, Cu) and sole solution of copper nanoparticles under the high pathogen infection background of Pseudocercosporella herpotrichoides (Fron) Deighton (synonym: Oculimacula yallundae (Wallwork & Sponer) Crous & W. Gams). It was shown that investigated nonionic colloidal solutions of biogenic metals have the antioxidant effect through the inhibition of the synthesis of lipid peroxidation products. The increase of lectin activity levels during the early plants ontogenesis stages was observed in wheat seedlings infected with pathogen pre-treated with the mixture of metal nanoparticles.

Keywords: Lectins; Lipid peroxidation; Metal nanoparticles; Oculimacula yallundae; Pseudocercosporella herpotrichoides; Wheat.

Fig. 1

TBARS content (nmol/g FW) in wheat seedlings at pre-sowing seed treatment with metal nanoparticles solutions and subsequent infection of plants with the suspension of P. herpotrichoides conidia. a control, b seed infection with P. herpotrichoides, c seed treatment with copper nanoparticles solution, d seed treatment with copper nanoparticles solution and subsequent seed infection with P. herpotrichoides, e seed treatment with metal nanoparticles (zinc, silver, iron, manganese, and copper) solution, f seed treatment with metal nanoparticles (zinc, silver, iron, manganese, and copper) solution and subsequent seed infection with P. herpotrichoides

Fig. 2

Dynamics of lectin activity ((mg/ml)⁻¹) in leaves of wheat seedlings in variants with seed treatment with the solutions of metal nanoparticles. a control, b seed treatment with copper nanoparticles solution, c seed treatment with metals nanoparticles solution (zinc, silver, iron, manganese, and copper)

Fig. 3

Dynamics of lectin activity ((mg/ml)^-1) in leaves of wheat seedlings in variants with seed treatment with the solutions of metal nanoparticles and the subsequent infection with the suspension of P. herpotrichoides conidia. a control, b seed infection with P. herpotrichoides, c seed treatment with copper nanoparticles solution and subsequent seed infection with P. herpotrichoides, d seed treatment with metal nanoparticle solution (zinc, silver, iron, manganese, and copper) and subsequent seed infection with P. herpotrichoides