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. 1989 Dec 22;246(4937):1595-7.
doi: 10.1126/science.2688091.

The anticodon contains a major element of the identity of arginine transfer RNAs

L H Schulman  1 , H Pelka
Affiliations

The anticodon contains a major element of the identity of arginine transfer RNAs

L H Schulman et al. Science. .

Abstract

The contribution of the anticodon to the discrimination between cognate and noncognate tRNAs by Escherichia coli Arg-tRNA synthetase has been investigated by in vitro synthesis and aminoacylation of elongator methionine tRNA (tRNA(mMet) mutants. Substitution of the Arg anticodon CCG for the Met anticodon CAU leads to a dramatic increase in Arg acceptance by tRNA(mMet). A nucleotide (A20) previously identified by others in the dihydrouridine loop of tRNA(Arg)s makes a smaller contribution to the conversion of tRNA(mMet) identity from Met to Arg. The combined anticodon and dihydrouridine loop mutations yield a tRNA(mMet) derivative that is aminoacylated with near-normal kinetics by the Arg-tRNA synthetase.

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