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Published Erratum
. 2016 Mar 1;113(9):E1328.
doi: 10.1073/pnas.1600609113. Epub 2016 Feb 16.

Correction for Kumar et al., Natural history-driven, plant-mediated RNAi-based study reveals CYP6B46's role in a nicotine-mediated antipredator herbivore defense

No authors listed
Published Erratum

Correction for Kumar et al., Natural history-driven, plant-mediated RNAi-based study reveals CYP6B46's role in a nicotine-mediated antipredator herbivore defense

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 3.
Fig. 3.
Effect of CYP6B46 silencing on larval nicotine excretion and nicotine flux in larval body. (A) Nicotine excreted (percent of total ingested) by fourth-instar EV- or irCYP-feeding larvae (experimental details in SI Appendix, Fig. S4) [(mean ± SE)]. (B) Nicotine in hemolymph of fourth-instar EV- or irCYP-feeding larvae (F1,4 = 106.6, P ≤ 0.01, n = 5). (C) Kinetics of nicotine absorption by (green arrows in larval body and green lines in graph) and discharge from (red arrows and red lines) the hemolymph of control and CYP-silenced fourth-instar larvae (experimental details in SI Appendix, Fig. S5A). (D) Kinetics of nicotine discharge from excised midguts (containing ingested host-plant diet) of control and CYP-silenced fourth instar larvae; nicotine entering the bathing solution (sodium phosphate buffer + 0.3 M sorbitol, pH 7.0) was measured at regular intervals, up to 60 min. (E) Kinetics of nicotine discharge from hemolymph of control and CYP-silenced fourth-instar larvae, after injecting 0.001% nicotine (of FM) into the hemolymph to determine if CYP-silenced larvae discharge nicotine from their hemolymph at rates different from that of controls. Asterisks indicate significant differences determined by one-way ANOVA (P ≤ 0.05). See Fig. 1 legend for the bar-shading codes.
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