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. 2015 Nov 15;8(11):21222-8.
eCollection 2015.

The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance

Songtao Xu  1 , Guojian Gu  2 , Qingfeng Ni  3 , Nan Li  4 , Kangkang Yu  4 , Xianjia Li  5 , Chang Liu  5
Affiliations

The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance

Songtao Xu et al. Int J Clin Exp Med. .

Abstract

Objective: Astrocyte elevated gene-1 (AEG-1) and Cyclin D1 is associated with tumorigenesis and progression. The aim of this study is to investigate the expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma (BUC) and explore their clinical and pathological significance.

Methods: The expression of AEG-1 and Cyclin D1 protein were detected in 85 cases of human BUC and 16 cases of tumor-adjacent tissues by the immunohistochemical method.

Results: The positive expression level of AEG-1 was 61.2% in human BUC which was higher than that in tumor-adjacent tissues (18.8%), P=0.002. The high expression of AEG-1 protein was correlated with the recurrence group (P=0.004). The positive rate of AEG-1 protein in superficial carcinoma group was lower than that of invasive cancer group (P=0.014). The positive expression level of Cyclin D1 was 56.5% in BUC, which was higher than that in tumor-adjacent tissues (12.5%). P=0.001. The high expression of Cyclin D1 protein was correlated with the recurrence group (P=0.024). The positive rate of Cyclin D1 protein in low grade group was lower than that of high grade group (P=0.001). The positive rate of Cyclin D1 protein in superficial carcinoma group was lower than that of invasive carcinoma group, (P=0.012). AEG-1 protein was positively correlated with Cyclin D1 protein (r=0.567, P<0.001). The log-rank test statistical analysis suggested that patients with higher AEG-1 or Cyclin D1 expression had shorter overall survival time, while patients with lower AEG-1 or Cyclin D1 expression had better survival.

Conclusion: Overexpression of AEG-1 and Cyclin D1 are markedly correlated with TNM stage and recurrence of BUC. Cyclin D1 are markedly correlated with grade of BUC. Detection of AEG-1 and Cyclin D1 may be helpful to evaluate prognosis and infiltrative capability of BUC.

Keywords: AEG-1; Bladder; Cyclin D1; carcinoma; immunohistochemistry.

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Figures

Figure 1
Figure 1
Immunohistochemical expression analysis of AEG-1 and Cyclin D1 protein in BUC tissue (200×). A. AEG-1 is positive staining in high grade of BUC tissue and its cytoplasm is stained brown. B. AEG-1 is negative staining in high grade of BUC tissue and its cytoplasm staining is weak. C. Cyclin D1 is positive staining in high grade of BUC tissue and its nucleus is stained brown. D. Cyclin D1 is negative staining in high grade of BUC tissue and its nucleus is not stained.
Figure 2
Figure 2
Kaplan-Meier survival curves of BUC patients based on AEG-1 expression (A) or Cyclin D1 expression (B).
See this image and copyright information in PMC

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