Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Nov 15;8(11):21442-7.
eCollection 2015.

Detection of microsatellite instability in gastric cancer and dysplasia tissues

Bing Li  1 , Hong-Yi Liu  1 , Shao-Hua Guo  1 , Peng Sun  1 , Fang-Ming Gong  1 , Bao-Qing Jia  1
Affiliations

Detection of microsatellite instability in gastric cancer and dysplasia tissues

Bing Li et al. Int J Clin Exp Med. .

Abstract

Objective: We aimed to investigate the association between gastric cancer and microsatellite instability (MSI) in the present study.

Method: Phenol-chloroform method was employed for DNA extraction from the cancer tissues of 65gastric cancer patients and the dysplasia tissues and normal control tissues of 32 non-gastric cancer patients. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250 were detected by using PCR-SSCP silver staining technique, and the MSI of the gastric cancer tissues and the precancerous tissues was analyzed.

Results: Of 65 gastric cancer cases, MSI was detected in 43 cases, with the detection rate of 66.2%. There were 13 cases showing MSI-H and 30 cases showing MSI-L, accounting for 30.2% and 69.8%, respectively. Among 32 cases of dysplasia tissues, MSI was detected in 10 cases, with the detection rate of 31.3%. Two cases of dysplasia tissues showed MSI-H and 8 cases showed MSI-L, accounting for 20.0% and 80.0%, respectively.

Conclusion: Gastric cancer patients had a high detection rate of MSI. It is speculated that MSI is another molecular mechanism of carcinogenesis and may serve as a sensitive diagnostic indicator of gastric cancer.

Keywords: Gastric cancer; PCR-SSCP analysis; microsatellite instability.

PubMed Disclaimer

Figures

Figure 1
Figure 1
PAG electrophoresis of 5 microsatellite loci, A: Bat25; B: Bat26; C: D5S346; D: D17S250; E: D2S123.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Jin Z, Jiang W, Wang L. Biomarkers for gastric cancer: Progression in early diagnosis and prognosis (Review) Oncol Lett. 2015;9:1502–1508. - PMC - PubMed
    1. Murphy A, Kelly RJ. From molecular classification to targeted therapeutics: the changing face of systemic therapy in metastatic gastroesophageal cancer. Gastroenterol Res Pract. 2015;2015:896560. - PMC - PubMed
    1. Zamanian-Azodi M, Rezaei-Tavirani M, Hasanzadeh H, Rahmati Rad S, Dalilan S. Introducing biomarker panel in esophageal, gastric, and colon cancers; a proteomic approach. Gastroenterol Hepatol Bed Bench. 2015;8:6–18. - PMC - PubMed
    1. Macdonald JS. Clinical overview: adjuvant therapy of gastrointestinal cancer. Cancer Chemother Pharmacol. 2004;54(Suppl 1):S4–11. - PubMed
    1. Simpson AJ, Caballero OL, Pena SD. Microsatellite instability as a tool for the classification of gastric cancer. Trends Mol Med. 2001;7:76–80. - PubMed

LinkOut - more resources