No systemic exposure of transtympanic heparin-binding epidermal growth factor like growth factor

Abstract

Context: Heparin-binding epidermal growth factor like growth factor (HB-EGF) is an emerging therapeutic for the regeneration of the tympanic membrane (TM).

Objective: Our aim was to determine whether the doses of HB-EGF delivered in a sustained release hydrogel into a middle ear mouse model, would be measurable in the systemic circulation. We also aimed to observe, in the scenario that the intended dose was absorbed directly into the circulation, whether these levels could be measured above the background levels of HB-EGF in the circulation.

Methods: A total of 12 mice had transtympanic injections of 5 μg/ml of HB-EGF contained within a previously described novel hydrogel vehicle, while another 12 mice had intravenous delivery of 10 μg/kg of HB-EGF. Intravenous blood samples were collected at 0-, 3-, 24-, 168-, 288- and 720-h post-injection. A double-antibody sandwich one-step process enzyme-linked immunosorbent assay (ELISA) was used to determine the level of HB-EGF in the serum.

Results: No mice in the transtympanic administration group and no mice in the intravenous administration group were found to have blood level measured above that in the controls.

Discussion: The inability of the positive control to measure levels above background, suggest the total dose used in our studies, even if 100% absorbed into the system circulation is insignificant.

Conclusions: HB-EGF at the doses and delivery method proposed for treatment of chronic TM perforation in a mouse model are likely to have no measurable systemic effect.

Keywords: Tympanic membrane; regeneration; tympanic perforation.

Figure 1

Figure 1 demonstrates the blood levels of HB-EGF as detected by ELISA following 5ug/ml trans tympanic delivery and 10ug/kg IV delivery. The negative control represents blood levels measured without any additional HB-EGF exposure. The positive control represents directly injecting 10ug/kg to a blood sample after collection from a non-HB-EGF treated mouse. The number of animal samples used per time point is indicated on each column. HB-EGF (heparin binding epidermal growth factor like growth factor), IV (intravenous), ELISA (enzyme-linked immunosorbent assay)