Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 May 18;7(4):395-9.
doi: 10.1080/21505594.2016.1149670. Epub 2016 Feb 18.

Novel real-time PCR assays for the specific detection of human infective Cryptosporidium species

Maha Bouzid  1 , Kristin Elwin  2 , Johanna L Nader  1 , Rachel M Chalmers  2 , Paul R Hunter  1 , Kevin M Tyler  1
Affiliations

Novel real-time PCR assays for the specific detection of human infective Cryptosporidium species

Maha Bouzid et al. Virulence. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Subtelomeric sequence data alignment at the 5′-end of chromosome 5 reveals significant sequence incongruence between C. hominis and C. parvum. Subtelomeric sequence data extending <30,000bp inwards from the 5′-telomeric end of chromosome 5 was extracted from newly-sequenced whole genome sequences for C. parvum subtype IIaA15G2R1 (UKP6) and C. hominis subtype IaA14R3 (UKH4). Sequences were aligned and compared using the Artemis Comparison Tool (ACT). Orthologous coding sequences were independently validated through The European Molecular Biology Open Software Suite (EMBOSS) Stretcher pairwise sequence alignment of nucleotide ORFs.
See this image and copyright information in PMC

References

    1. Baldursson S, Karanis P. Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2004–2010. Water Res 2011; 45:6603-14; PMID:22048017; http://dx.doi.org/10.1016/j.watres.2011.10.013 - DOI - PubMed
    1. Bouzid M, Hunter PR, Chalmers RM, Tyler KM. Cryptosporidium Pathogenicity and Virulence. Clin Microbiol Rev 2013; 26:115-34; PMID:23297262; http://dx.doi.org/10.1128/CMR.00076-12 - DOI - PMC - PubMed
    1. Hunter PR, Hughes S, Woodhouse S, Raj N, Syed Q, Chalmers RM, Verlander NQ, Goodacre J. Health sequelae of human cryptosporidiosis in immunocompetent patients. Clin Infect Dis 2004; 39:504-10; PMID:15356813; http://dx.doi.org/10.1086/422649 - DOI - PubMed
    1. Puleston RL, Mallaghan CM, Modha DE, Hunter PR, Nguyen-Van-Tam JS, Regan CM, Nichols GL, Chalmers RM. The first recorded outbreak of cryptosporidiosis due to Cryptosporidium cuniculus (formerly rabbit genotype), following a water quality incident. J Water Health 2014; 12:41-50; PMID:24642431; http://dx.doi.org/10.2166/wh.2013.097 - DOI - PubMed
    1. Bouzid M, Steverding D, Tyler KM. Detection and surveillance of waterborne protozoan parasites. Curr Opin Biotech 2008; 19:302-6; PMID:18524569; http://dx.doi.org/10.1016/j.copbio.2008.05.002 - DOI - PubMed

MeSH terms