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. 2016 Feb 20:2016:bav120.
doi: 10.1093/database/bav120. Print 2016.

The UniProtKB guide to the human proteome

Lionel Breuza  1 , Sylvain Poux  2 , Anne Estreicher  2 , Maria Livia Famiglietti  2 , Michele Magrane  3 , Michael Tognolli  2 , Alan Bridge  2 , Delphine Baratin  2 , Nicole Redaschi  2 ; UniProt Consortium
Affiliations

The UniProtKB guide to the human proteome

Lionel Breuza et al. Database (Oxford). .

Abstract

Advances in high-throughput and advanced technologies allow researchers to routinely perform whole genome and proteome analysis. For this purpose, they need high-quality resources providing comprehensive gene and protein sets for their organisms of interest. Using the example of the human proteome, we will describe the content of a complete proteome in the UniProt Knowledgebase (UniProtKB). We will show how manual expert curation of UniProtKB/Swiss-Prot is complemented by expert-driven automatic annotation to build a comprehensive, high-quality and traceable resource. We will also illustrate how the complexity of the human proteome is captured and structured in UniProtKB. Database URL: www.uniprot.org.

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Figures

Figure 1.
Figure 1.
Accessing the human proteome from the UniProt web site (http://www.uniprot.org). A. One can directly type ‘HUMAN’ in the search box. B. Then select ‘Human’ in the ‘Popular organisms’ section on the left.C. There is a single proteome in the ‘Proteomes’ section for this organism, UP000005640, a direct link allowing access to the entries composing the human complete proteome. D. There, one still has the possibility to select the 20 197 expertly ‘Reviewed’ entries of the Swiss-Prot section of UniProtKB from the 49 496 additional ‘unreviewed’ entries of UniProtKB/TrEMBL.
Figure 2.
Figure 2.
Screenshot of the ‘Function’ and ‘Pathology and Biotech’ sections of human FAM20A entry (UniProtKB Q96MK3, http://www.uniprot.org/uniprot/Q96MK3).
Figure 3.
Figure 3.
Annotation scheme for integration of proteomics data in UniProtKB.
See this image and copyright information in PMC

References

    1. Tennessen J.A., Bigham A.W., O’Connor T.D. et al. (2012) Evolution and functional impact of rare coding variation from deep sequencing of human exomes. Science, 337, 64–69. - PMC - PubMed
    1. Kim M.S., Pinto S.M., Getnet D. et al. (2014) A draft map of the human proteome. Nature, 509, 575–581. - PMC - PubMed
    1. Wilhelm M., Schlegl J., Hahne H. et al. (2014) Mass-spectrometry-based draft of the human proteome. Nature, 509, 582–587. - PubMed
    1. Wan C., Borgeson B., Phanse S. et al. (2015) Panorama of ancient metazoan macromolecular complexes. Nature, 525, 339–344. - PMC - PubMed
    1. Ezkurdia I., Vazquez J., Valencia A. et al. (2014) Analyzing the first drafts of the human proteome. J. Proteome Res., 13, 3854–3855. - PMC - PubMed

Publication types