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. 2016 Feb 20:12:31.
doi: 10.1186/s12917-016-0656-1.

Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

Renata Duarte da Silva Cezar  1 , Norma Lucena-Silva  2 , Antônio Fernando Barbosa Batista Filho  3 , Jonas de Melo Borges  4 , Pollyane Raysa Fernandes de Oliveira  5 , Érica Chaves Lúcio  6 , Maíra Arruda-Lima  7 , Vania Lucia de Assis Santana  8 , José Wilton Pinheiro Junior  9
Affiliations

Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

Renata Duarte da Silva Cezar et al. BMC Vet Res. .

Abstract

Background: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated.

Results: Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB.

Conclusions: M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil.

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