doi: 10.1128/mcb.9.11.5012-5021.1989.
Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes
Affiliations
- PMID: 2689871
- PMCID: PMC363653
- DOI: 10.1128/mcb.9.11.5012-5021.1989
Item in Clipboard
Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes
Mol Cell Biol.
1989 Nov.
Abstract
The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.
Similar articles
-
Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes.Mol Cell Biol. 1990 Dec;10(12):6257-63. doi: 10.1128/mcb.10.12.6257-6263.1990. Mol Cell Biol. 1990. PMID: 1978925 Free PMC article.
-
High-level expression and simplified purification of recombinant ricin A chain.Protein Expr Purif. 1992 Oct;3(5):386-94. doi: 10.1016/s1046-5928(05)80040-3. Protein Expr Purif. 1992. PMID: 1458052
-
Mutagenesis and kinetic analysis of the active site Glu177 of ricin A-chain.Protein Eng. 1993 Jun;6(4):425-31. doi: 10.1093/protein/6.4.425. Protein Eng. 1993. PMID: 8332600
-
Chemical and genetic characterization of the enzymatic activity associated with ricin A chain.Targeted Diagn Ther. 1992;7:151-82. Targeted Diagn Ther. 1992. PMID: 1633288 Review. No abstract available.
-
Cytotoxic ribosome-inactivating lectins from plants.Biochim Biophys Acta. 2004 Sep 1;1701(1-2):1-14. doi: 10.1016/j.bbapap.2004.06.004. Biochim Biophys Acta. 2004. PMID: 15450171 Review.
Cited by
-
Alteration of an amino acid residue outside the active site of the ricin A chain reduces its toxicity towards yeast ribosomes.Mol Gen Genet. 1991 Nov;230(1-2):81-90. doi: 10.1007/BF00290654. Mol Gen Genet. 1991. PMID: 1745246
-
Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome.Infect Immun. 2016 Nov 18;84(12):3290-3301. doi: 10.1128/IAI.00630-16. Print 2016 Dec. Infect Immun. 2016. PMID: 27600507 Free PMC article.
-
Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4869-73. doi: 10.1073/pnas.89.11.4869. Proc Natl Acad Sci U S A. 1992. PMID: 1594586 Free PMC article.
-
An enzymatic mutant of Shiga-like toxin II variant is a vaccine candidate for edema disease of swine.Infect Immun. 1992 Feb;60(2):485-90. doi: 10.1128/iai.60.2.485-490.1992. Infect Immun. 1992. PMID: 1730480 Free PMC article.
-
Exploring steric constraints on protein mutations using MAGE/PROBE.Protein Sci. 2000 Nov;9(11):2251-9. doi: 10.1110/ps.9.11.2251. Protein Sci. 2000. PMID: 11152136 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
