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. 2016 Feb 12:7:139.
doi: 10.3389/fpls.2016.00139. eCollection 2016.

Glutathione S-Transferase Gene Family in Gossypium raimondii and G. arboreum: Comparative Genomic Study and their Expression under Salt Stress

Yating Dong  1 , Cong Li  1 , Yi Zhang  1 , Qiuling He  1 , Muhammad K Daud  2 , Jinhong Chen  1 , Shuijin Zhu  1
Affiliations

Glutathione S-Transferase Gene Family in Gossypium raimondii and G. arboreum: Comparative Genomic Study and their Expression under Salt Stress

Yating Dong et al. Front Plant Sci. .

Abstract

Glutathione S-transferases (GSTs) play versatile functions in multiple aspects of plant growth and development. A comprehensive genome-wide survey of this gene family in the genomes of G. raimondii and G. arboreum was carried out in this study. Based on phylogenetic analyses, the GST gene family of both two diploid cotton species could be divided into eight classes, and approximately all the GST genes within the same subfamily shared similar gene structure. Additionally, the gene structures between the orthologs were highly conserved. The chromosomal localization analyses revealed that GST genes were unevenly distributed across the genome in both G. raimondii and G. arboreum. Tandem duplication could be the major driver for the expansion of GST gene families. Meanwhile, the expression analysis for the selected 40 GST genes showed that they exhibited tissue-specific expression patterns and their expression were induced or repressed by salt stress. Those findings shed lights on the function and evolution of the GST gene family in Gossypium species.

Keywords: GST; cotton; gene family; phylogenetic analysis; salt stress.

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Figures

Figure 1
Figure 1
Phylogenetic relationships of GST genes from G. raimondii, Arabidopsis, and rice. The unrooted phylogentic tree was constructed using MEGA 5.2 by Neighbor-Joining method and the bootstrap test was performed with 1000 replicates. Percentage bootstrap scores of >50% were displayed. The GST genes from G. raimondii, Arabidopsis and rice were marked with the red dots, green triangles, and blue rhombuses respectively. And the branches of each subfamily were indicated in a specific color.
Figure 2
Figure 2
Phylogenetic relationships of GST genes from G. arboreum, Arabidopsis, and rice. The unrooted phylogentic tree was constructed using MEGA 5.2 by Neighbor-Joining method and the bootstrap test was performed with 1000 replicates. Percentage bootstrap scores of >50% were displayed. The GST genes from G. arboreum, Arabidopsis and rice were marked with the red dots, green triangles, and blue rhombuses, respectively. And the branches of each subfamily were indicated in a specific color.
Figure 3
Figure 3
Phylogenetic relationships and gene structure of GST genes from G. raimondii and G. arboreum. (A) The phylogenetic tree of all GST genes in G. raimondii and G. arboreum was constructed using MEGA 5.2 by Neighbor-Joining method and the bootstrap test was performed with 1000 replicates. Percentage bootstrap scores of >50% were displayed. The GST genes from G. raimondii and G. arboreum were marked with red dots and blue dots, respectively. Gene names in gray background shown orthologous pairs. (B) The exon-inton structure of GST genes from G. raimondii and G. arboreum. Exons were represented by green boxes and introns by gray lines.
Figure 4
Figure 4
Chromosomal distribution and gene duplication of GST genes in G. raimondii. The chromosome number was indicated at the top of each chromosome representation. The scale on the left was in megabases (Mb). The genes with a pentagram left represent GST gene clusters. The tandem duplicated genes were highlighted with outlined boxes. And the segmental duplicated gene pairs are connected with red lines.
Figure 5
Figure 5
Chromosomal distribution and gene duplication of GST genes in G. arboreum. The chromosome number was indicated at the top of each chromosome representation. The scale on the left was in megabases (Mb). The genes with a pentagram left represent GST gene clusters. Red outlined boxes represent tandem duplicated genes. And the segmental duplicated gene pairs were connected with red lines.
Figure 6
Figure 6
Expression patterns of 40 selected GST genes in four representative tissues of G. raimondii and G. arboreum. The color bar represents the relative signal intensity values.
Figure 7
Figure 7
Expression patterns of 40 selected GST genes in four representative tissues of G. raimondii and G. arboreum under salt stress. The color bar represents the relative signal intensity values. The slight stress, moderate stress, and severe stress represent 50, 100, and 200 mM NaCl in G. raimondii and 100, 200, and 300 mM NaCl in G. arboreum, respectively.
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References

    1. Armstrong R. N. (1997). Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem. Res. Toxicol. 10, 2–18. 10.1021/tx960072x - DOI - PubMed
    1. Bjellqvist B., Basse B., Olsen E., Celis J. E. (1994). Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions. Electrophoresis 15, 529–539. 10.1002/elps.1150150171 - DOI - PubMed
    1. Blanc G., Wolfe K. H. (2004). Widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes. Plant Cell 16, 1667–1678. 10.1105/tpc.021345 - DOI - PMC - PubMed
    1. Campo S., Baldrich P., Messeguer J., Lalanne E., Coca M., Segundo B. S. (2014). Overexpression of a calcium-dependent protein kinase confers salt and drought tolerance in rice by preventing membrane lipid peroxidation. Plant Physiol. 165, 688–704. 10.1104/pp.113.230268 - DOI - PMC - PubMed
    1. Chan C., Lam H. M. (2014). A putative lambda class glutathione S-transferase enhances plant survival under salinity stress. Plant Cell Physiol. 55, 570–579. 10.1093/pcp/pct201 - DOI - PubMed