Expression data on liver metabolic pathway genes and proteins

Abstract

Here, we present the expression data on various metabolic pathways of liver with special emphasize on lipid and carbohydrate metabolism and long chain polyunsaturated fatty acid (PUFA) synthesis, both at gene and protein levels. The data were obtained to understand the effect of vitamin A deficiency on the expression status (both gene and protein levels) of some of the key factors involved in lipogenesis, fatty acid oxidation, triglyceride secretion, long chain PUFA, resolvin D1 synthesis, glucose transport and glycogen synthesis of liver, using modern biology tools, such as quantitative real-time PCR (RT-PCR) and immunoblotting techniques. This data article provides the supporting evidence to the article "Vitamin A deficiency suppresses high fructose-induced triglyceride synthesis and elevates resolvin D1 levels" [1] and therefore, these data may be referred back, for comprehensive understanding and interpretations and for future studies.

Keywords: Glycogen; Lipid; PUFA; Resolvin; Steatosis; Vitamin A.

Fig. S1

Fig. 1

Gene expression of liver metabolic pathways. Relative mRNA levels normalized to control. Data are expressed as mean±SEM (4–5 rats from each group). Data were analyzed by one way ANOVA with post-hoc least significant difference (post-hoc LSD) test. ^$ Significant, when compared to control at P≤0.05 level. P values ≤0.05 and 0.01 levels were denoted as * and ** respectively.

Fig. 2

Protein expression of liver metabolic pathways. Representative immunoblots with densitometric analyses data. Data are expressed as mean±SEM (3–4 rats from each group). Data were analyzed by one way ANOVA with post-hoc least significant difference (post-hoc LSD) test. * Significant at P value ≤0.05 level.